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Product name
TMRE—Mitochondrial Membrane Potential Assay Kit
See all Mitochondrial Transmembrane Potential products (8) ...
Product overview
ab113852 is suitable for interrogating mitochondrial membrane potential in live cells for analysis by flow cytometry, microplate spectrophotometry and fluorescent microscopy. Each assay kit contains sufficient materials for at least 200 measurements.
ab113852 uses TMRE (tetramethylrhodamine, ethyl ester) to label active mitochondria. TMRE is a cell permeant, positively-charged, red-orange dye that readily accumulates in active mitochondria due to their relative negative charge. Depolarized or inactive mitochondria have decreased membrane potential and fail to sequester TMRE.
FCCP (carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone) is a ionophore uncoupler of oxidative phosphorylation. Treating cells with FCCP eliminates mitochondrial membrane potential and TMRE staining. TMRE is suitable for the labeling of mitochondria in live cells and is not compatible with fixation.
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Please refer to protocols.
| Components | Identifier | 100 tests |
|---|---|---|
| 1mM TMRE (in DMSO) | P/N 8206010 | 1 x 40µl |
| 50mM FCCP (in DMSO) | P/N 8206009 | 1 x 10µl |
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Kits/ Lysates/ Other >> Kits >> Cell Damage Kits >> Cell viability, plasma membrane damage
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Cell Viability and Senescence Kits
Kits/ Lysates/ Other >> Kits >> Apoptosis Kits >> Other Apoptosis Kits
Kits/ Lysates/ Other >> Kits >> Cell Damage Kits
Cell Biology >> Apoptosis >> Mitochondrial
Relevance
Mitochondrial Transmembrane Potential (? Ψ) is an important parameter of mitochondrial function used as an indicator of cell death. The collpase of the (??) mitochondrial transmembrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.
Alternative names
- MS960
Applications
Show applications key
Our Abpromise guarantee covers the use of ab113852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS
TMRE—Mitochondrial Membrane Potential Assay Kit images:
Functional Studies - TMRE—Mitochondrial Membrane Potential Assay Kit (ab113852)
Microplate analysis of cells showing mean fluorescent intensity +/- standard deviation from quadruplicate measurements of 400nM ab113852 (TMRE) stained Jurkat cells in a 96-well microplate +/- treatment with FCCP.
Immunocytochemistry/ Immunofluorescence - TMRE—Mitochondrial Membrane Potential Assay Kit (ab113852)
A: HeLa cells (adherent) were cultured on coverslips and stained with ab113852 (200nM TMRE) for 20 minutes in media, washed briefly with PBS and immediately imaged. B: Jurkat cells (suspension) were stained and washed as above and then transferred to a slide and immobilized under a coverslip for imaging.
Flow Cytometry - TMRE—Mitochondrial Membrane Potential Assay Kit (ab113852)
Flow cytometry histogram of Jurkat cells stained with ab113852 (100nM TMRE) with (blue) or without (red) treatment with 100nM FCCP.
Protocols
References for TMRE—Mitochondrial Membrane Potential Assay Kit (ab113852)
ab113852 has not yet been referenced specifically in any publications.
Publishing research using ab113852? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"