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Product name Anti-TMS1 antibody See all TMS1 primary antibodies
Goat polyclonal to TMS1
Specificity This antibody is expected to recognize all three reported isforms (NP_037390; NP_660183; NP_660184).
Tested applications Suitable for:
IHC-P, WB more details
Synthetic peptide: RESQSYLVEDLERS, corresponding to amino acids 182-195 of Human TMS1.
HeLa cell lysate
Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
Purity Immunogen affinity purified
Purification notes Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).Can be blocked with
Human TMS1 peptide (ab22875) or TMS1 peptide (ab8395).
Function Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.
Tissue specificity Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells.
Sequence similarities Contains 1 CARD domain. Contains 1 DAPIN domain.
Domain Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain.
Post-translational modifications Phosphorylated.
Cellular localization Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures.
Information by UniProt
Apoptosis associated speck like protein containing a CARD antibody
Apoptosis-associated speck-like protein containing a CARD antibody
Anti-TMS1 antibody images
References for Anti-TMS1 antibody (ab2236)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"