Overview

  • Product nameAnti-TMS1 antibody
    See all TMS1 primary antibodies
  • Description
    Goat polyclonal to TMS1
  • SpecificityThis antibody is expected to recognize all three reported isforms (NP_037390; NP_660183; NP_660184).
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: RESQSYLVEDLERS, corresponding to amino acids 182-195 of Human TMS1.

  • Positive control
    • HeLa cell lysate Human Liver

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab2236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).Can be blocked with Human TMS1 peptide (ab22875) or TMS1 peptide (ab8395).

Target

  • FunctionPromotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.
  • Tissue specificityWidely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells.
  • Sequence similaritiesContains 1 CARD domain.
    Contains 1 DAPIN domain.
  • DomainInteracts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localizationCytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis associated speck like protein containing a CARD antibody
    • Apoptosis-associated speck-like protein containing a CARD antibody
    • ASC antibody
    • ASC_HUMAN antibody
    • CARD 5 antibody
    • CARD5 antibody
    • Caspase recruitment domain containing protein 5 antibody
    • Caspase recruitment domain protein 5 antibody
    • Caspase recruitment domain-containing protein 5 antibody
    • hASC antibody
    • MGC10332 antibody
    • PYCARD antibody
    • PYD and CARD domain containing antibody
    • PYD and CARD domain containing protein antibody
    • PYD and CARD domain-containing protein antibody
    • Target of methylation induced silencing 1 antibody
    • Target of methylation-induced silencing 1 antibody
    • TMS 1 antibody
    • TMS antibody
    • TMS1 antibody
    see all

Anti-TMS1 antibody images

  • Anti-TMS1 antibody (ab2236) at 1 µg/ml + Cell lysate prepared from human Hella cells at 35 µg
    Developed using the ECL technique

    Predicted band size : 22 kDa
    Observed band size : 22 kDa


    Primary incubated for 1 hour.

  • ab2236 at 2.5 µg/ml staining TMS1 in Human liver by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References for Anti-TMS1 antibody (ab2236)

ab2236 has not yet been referenced specifically in any publications.

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