TMS1 overexpression 293T lysate (whole cell) (ab94129)

Overview

  • Product nameTMS1 overexpression 293T lysate (whole cell)
    See all TMS1 lysates
  • Description
    TMS1 overexpression lysate
  • General notesab94129 is a 293T cell transfected lysate in which Human TMS1 has been transiently over-expressed using a pCMV-TMS1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Tissue specificity: Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells. Similarity: Contains 1 CARD domain. Contains 1 DAPIN domain. Domain: Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain. PTM: Phosphorylated.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

TMS1 overexpression 293T lysate (whole cell) images

  • ab94129 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-TMS1 antibody (ab89611) at 1/500 dilution

    Lane 1 : TMS1 overexpression 293T lysate (whole cell) (ab94129)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for TMS1 overexpression 293T lysate (whole cell) (ab94129)

ab94129 has not yet been referenced specifically in any publications.

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