Overview

  • Product name
    Anti-TMTC4 antibody
  • Description
    Rabbit polyclonal to TMTC4
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (NKDLQAETPL GDLWHHDFWG SRLSSNTSHK SYRPLTVLTF RINYYLSGGF) of Human TMTC4 (NP_116202).

  • Positive control
    • MCF7 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

  • Relevance
    TMTC4 belongs to the TMTC family. Its exact function remains unknown.
  • Cellular localization
    Membrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • FLJ14624 antibody
    • FLJ22153 antibody
    • OTTHUMP00000018648 antibody
    • OTTHUMP00000018649 antibody
    • OTTHUMP00000018650 antibody
    • OTTHUMP00000018651 antibody
    • OTTHUMP00000018652 antibody
    • TMTC 4 antibody
    • Transmembrane and tetratricopeptide repeat containing 4 antibody
    • Transmembrane and TPR repeat containing protein 4 antibody
    see all

Images

  • Anti-TMTC4 antibody (ab83730) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 83 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling TMTC4 with ab83730 at 4-8µg/ml.

References

ab83730 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab83730.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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