Recombinant Anti-TMUB2 antibody [EPR13741] (ab181183)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13741] to TMUB2
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TMUB2 antibody [EPR13741]
See all TMUB2 primary antibodies -
Description
Rabbit monoclonal [EPR13741] to TMUB2 -
Host species
Rabbit -
Specificity
Based on the sequence analysis, ab181183 recognizes 4 isoforms with predicted MW of 34KDa, 32KDa, 19KDa and 10KDa, respectively. -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, HEK293, HepG2 and A431 cell lysates. HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13741 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/150.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/2000. Detects a band of approximately 10-34 kDa (predicted molecular weight: 34 kDa).
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IP |
1/30 - 1/50.
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Notes |
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Flow Cyt (Intra)
1/150. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/2000. Detects a band of approximately 10-34 kDa (predicted molecular weight: 34 kDa). |
IP
1/30 - 1/50. |
Target
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Sequence similarities
Contains 1 ubiquitin-like domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 79089 Human
- SwissProt: Q71RG4 Human
- Unigene: 181391 Human
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Alternative names
- FP2653 antibody
- MGC3123 antibody
- Tmub2 antibody
see all
Images
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All lanes : Anti-TMUB2 antibody [EPR13741] (ab181183) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HEK293 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 10-34 kDa why is the actual band size different from the predicted? -
Intracellular Flow Cytometry analysis of TMUB2 expression in HeLa cells using ab181183 at 1/150 dilution (red) and a rabbit IgG as negative control (green).
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Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (lane 1) or negative control (lane 2), labeling TMUB2 immunoprecipitated using ab181183 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181183 has not yet been referenced specifically in any publications.