Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab9579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt Use 1-2µg for 106 cells.
WB Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
IHC-P 1/100.
IHC-Fr Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

Target

  • FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localizationSecreted and Cell membrane.
  • Target information above from: UniProt accession P01375 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • MCF antibody
    • Necrosin antibody
    • TNF a antibody
    • TNF alpha antibody
    • TNF antibody
    • TNF Macrophage Derived antibody
    • TNF Monocyte Derived antibody
    • TNF Superfamily Member 2 antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor Precursor antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    • Tumour Necrosis Factor Alpha antibody
    see all

Anti-TNF alpha antibody [P/T2] images

  • The antibody ab9579 labels the cytoplasm of TNF-a secreting cells including histiocytes/macrophages.
  • Overlay histogram showing THP1 cells stained with ab9579 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9579, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemisty of Human adenocarcinoma uterum (with positive reactive macrophages).
  • Immunochistochemistry of Human mandibular cyst (granulomatous inflammation with positive macrophages).

References for Anti-TNF alpha antibody [P/T2] (ab9579)

This product has been referenced in:
  • Cui X  et al. Resveratrol suppresses colitis and colon cancer associated with colitis. Cancer Prev Res (Phila Pa) 3:549-59 (2010). IHC-P ; Mouse . Read more (PubMed: 20332304) »
  • Emura I & Usuda H Histopathological and cytological examination of autopsy cases with multiple organ dysfunction syndromes. Pathol Int 60:443-51 (2010). ICC ; Human . Read more (PubMed: 20518899) »

See all 8 Publications for this product

Product Wall

Thank you for your enquiry. I have attached a copy of the IHC image that is on the datasheet for the antibody, a granulomatous inflammatory reaction, probably in a chronic cholecystitis. The species is human. Please let me know if you have any questions.

Thank you for your inquiry.

I am sorry to hear that your customer is having trouble with these antibodies.

It seems to me that there must be an issue with the HRP polymer detection system, DAB,or antigen retrieval buffers/system. Th...

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Thank you for contacting us. I have received the following information from the lab: The antigen retrieval performed with this antibody in paraffin sections is: 5 minutes heat-induced epitope retrieval (HIER) in boiling citrate buffer, pH 6.0....

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Thank you for your enquiry. From the three antibodies that you list I would like to recommend TNF alpha antibody (ab6671). This antibody has received a favourable Abreview from one of our customers detailing its application following citrate antige...

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Thank you for your enquiry. The best positive control for this antibody is human macrophages in a granulomatous inflammatory lesion. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Thank you for your enquiry. I am sorry to hear that you have been experiencing problems with ab8999. As discussed on the telephone this afternoon please would you fill in the questionnaire by clicking on the link below. I would also appreciate it i...

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Thank you for your email. Did you order the antibody directly from Abcam or from one a distributor, such as Biozol Diagnostica? If you ordered from a distributor, please indicate which one and also the purchase order number that you used with them. The...

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Thank you for your message. I have checked with the originator of this antibody and there have not been any complaints concerning this lot. I'm still waiting to obtain some more information regarding the use of this antibody in Western blotting, but I ...

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Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with this antibody. I would like to make a few suggestions to try to help you out. To see if you can obtain a signal, try incubating with the primary antibody overnig...

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Yes, ab9570 will work using Envision + mouse and/or Envision mouse-rabbit kit.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"