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Abcam’s TNF alpha mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TNF alpha in mouse serum, buffered solutions and cell culture medium.
A monoclonal antibody specific for TNF alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TNF alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TNF alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TNF alpha present in the samples.
This kit will recognize both endogenous and recombinant mouse TNF alpha.
|Components||Identifier||1 x 96 tests||2 x 96 tests|
|10X Standard Diluent Buffer||Black||1 x 25ml||1 x 25ml|
|200X Wash Buffer||White||1 x 10ml||2 x 10ml|
|Biotinylated Antibody Diluent||Red||1 x 7.5ml||1 x 13ml|
|Biotinylated anti-TNFalpha||Red||1 x 400µl||2 x 400µl|
|Chromogen TMB Substrate Solution||1 x 11ml||1 x 24ml|
|HRP Diluent||Red||1 x 23ml||1 x 23ml|
|Stop Reagent||Black||1 x 11ml||2 x 11ml|
|Streptavidin HRP||2 x 5µl||4 x 5µl|
|TNF alpha Microplate (12 x 8 well strips)||1 unit||2 units|
|TNF alpha Standard (lyophilized)||Yellow||2 vials||4 vials|
Our Abpromise guarantee covers the use of ab46105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"