The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.25µg for 105-8 cells. A 100 µl volume is recommended for staining the cell sample.
Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.
Involvement in disease
Genetic variations in LTA are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
Belongs to the tumor necrosis factor family.
Secreted. Membrane. The homotrimer is secreted. The heterotrimer is membrane-associated.
Yokley BH et al. A stimulation-dependent alternate core promoter links lymphotoxin a expression with TGF-ß1 and fibroblast growth factor-7 signaling in primary human T cells. J Immunol190:4573-84 (2013).
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