Anti-TNF beta antibody [AT15A3] (ab100844)

Overview

  • Product name
    Anti-TNF beta antibody [AT15A3]
    See all TNF beta primary antibodies
  • Description
    Mouse monoclonal [AT15A3] to TNF beta
  • Tested applications
    Suitable for: IHC-P, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 35-205 of Human TNF beta (NP_001153212)

  • Positive control
    • HeLa cells and Mouse Spleen lysate IHC-P: Human skin melanoma FFPE tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab100844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500. Predicted molecular weight: 22 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.

Target

  • Function
    Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.
  • Involvement in disease
    Genetic variations in LTA are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Cellular localization
    Secreted. Membrane. The homotrimer is secreted. The heterotrimer is membrane-associated.
  • Information by UniProt
  • Database links
  • Alternative names
    • LTalpha antibody
    • DIF antibody
    • LT alpha antibody
    • LT antibody
    • LT-alpha antibody
    • Lta antibody
    • Lymphotoxin alpha (TNF superfamily, member 1) antibody
    • Lymphotoxin alpha antibody
    • Lymphotoxin-alpha antibody
    • TNF B antibody
    • TNF superfamily member 1 antibody
    • TNF, lymphocyte-derived antibody
    • TNF-beta antibody
    • TNFB antibody
    • TNFB_HUMAN antibody
    • TNFbeta antibody
    • TNFSF1 antibody
    • TNLG1E antibody
    • Tumor necrosis factor beta antibody
    • tumor necrosis factor ligand 1E antibody
    • Tumor necrosis factor ligand superfamily member 1 antibody
    see all

Images

  • IHC image of ab100844 staining in human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab100844, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-TNF beta antibody [AT15A3] (ab100844) at 1/1000 dilution + Mouse Spleen Tissue Lysate at 35 µg

    Secondary
    goat anti-mouse conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 22 kDa
  • Immunofluorescence of Human HeLa cells stained with ab100844 at 1/500 with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).

References

ab100844 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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This antibody has been tested in indirect ELISA only; we are unsure about the sandwich ELISA pair. The epitope of this antibody has also not been mapped which is another obstacle for finding a matched pair based on...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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