The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa.
1/1000. Antigen retrieval is not essential but may optimise staining.
Incubate for 1 hour at RT.
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration. PubMed: 17210691
Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
Involvement in disease
Familial hibernian fever Multiple sclerosis 5
Contains 1 death domain. Contains 4 TNFR-Cys repeats.
The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE. Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
The soluble form is produced from the membrane form by proteolytic processing.
Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
Tumor necrosis factor receptor superfamily, member 1A antibody
Tumor necrosis factor receptor type 1 antibody
Tumor necrosis factor receptor type I antibody
Tumor necrosis factor-binding protein 1 antibody
Western blot - Anti-TNF Receptor I antibody (ab19139)
Western blot analysis of mouse RAW 264 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF Receptor I antibody (ab19139)
Ab19139 staining human normal placenta. Staining is localized to cell membrane and secreted. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Western blot - Anti-TNF Receptor I antibody (ab19139)This image is courtesy of an anonymous Abreview
Anti-TNF Receptor I antibody (ab19139) at 1/1000 dilution + Human 293T whole cell lysate at 30000 cells
Secondary An HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Flow Cytometry - Anti-TNF Receptor I antibody (ab19139)Image courtesy of an anonymous Abreview.
ab19139 staining TNF Receptor I in Human platelet cells by Flow cytometry. Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/100 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.
Zhang C et al. Activation of TNF-a/NF-?B axis enhances CRL4BDCAF11E3 ligase activity and regulates cell cycle progression in human osteosarcoma cells. Mol OncolN/A:N/A (2018).
Read more (PubMed: 29377600) »
Wen S et al. Necroptosis is a key mediator of enterocytes loss in intestinal ischaemia/reperfusion injury. J Cell Mol Med21:432-443 (2017).
Read more (PubMed: 27677535) »