Anti-TNF Receptor I antibody (ab90463)

Overview

  • Product name
    Anti-TNF Receptor I antibody
    See all TNF Receptor I primary antibodies
  • Description
    Rabbit polyclonal to TNF Receptor I
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Human, Pig, Saccharomyces cerevisiae, Xenopus laevis, Drosophila melanogaster, Monkey
  • Immunogen

    Synthetic peptide derived from the sequence of mouse TNF Receptor 1

  • Positive control
    • Mouse brain, Rat brain, HeLa (heat shocked) or Jurkat membrane lysate

Applications

Our Abpromise guarantee covers the use of ab90463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at a concentration of 12.5 µg/ml.
    WB (ECL): 1/1000. Predicted molecular weight: 51 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
    • Involvement in disease
      Familial hibernian fever
      Multiple sclerosis 5
    • Sequence similarities
      Contains 1 death domain.
      Contains 4 TNFR-Cys repeats.
    • Domain
      The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
      Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
    • Post-translational
      modifications
      The soluble form is produced from the membrane form by proteolytic processing.
    • Cellular localization
      Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD120a antibody
      • FPF antibody
      • MGC19588 antibody
      • p55 antibody
      • p55-R antibody
      • p60 antibody
      • TBP1 antibody
      • TBPI antibody
      • TNF R antibody
      • TNF R55 antibody
      • TNF-R1 antibody
      • TNF-RI antibody
      • TNFAR antibody
      • TNFR-I antibody
      • TNFR1 antibody
      • TNFR55 antibody
      • TNFR60 antibody
      • TNFRI antibody
      • TNFRSF1a antibody
      • TNR1A_HUMAN antibody
      • Tumor necrosis factor receptor 1 antibody
      • Tumor necrosis factor receptor superfamily, member 1A antibody
      • Tumor necrosis factor receptor type 1 antibody
      • Tumor necrosis factor receptor type I antibody
      • Tumor necrosis factor-binding protein 1 antibody
      see all

    Images

    • All lanes : Anti-TNF Receptor I antibody (ab90463) at 1/1000 dilution

      Lane 1 : HeLa (heat shocked) cell extract
      Lane 2 : Jurkat cell extract


      Predicted band size : 51 kDa

    References

    ab90463 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain - Striatum)
    Permeabilization
    No
    Specification
    Brain - Striatum
    Blocking step
    TNB as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Vanessa Johanssen

    Verified customer

    Submitted Apr 13 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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