The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at a concentration of 12.5 µg/ml.
WB (ECL): 1/1000. Predicted molecular weight: 51 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
Involvement in disease
Familial hibernian fever Multiple sclerosis 5
Contains 1 death domain. Contains 4 TNFR-Cys repeats.
The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE. Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
The soluble form is produced from the membrane form by proteolytic processing.
Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
Zhou Z et al. Inhibition of Epithelial TNF-a Receptors by Purified Fruit Bromelain Ameliorates Intestinal Inflammation and Barrier Dysfunction in Colitis. Front Immunol8:1468 (2017).
Read more (PubMed: 29176974) »