Anti-TNF Receptor I (phospho S274) antibody (ab60031)

Overview

  • Product name
    Anti-TNF Receptor I (phospho S274) antibody
    See all TNF Receptor I primary antibodies
  • Description
    Rabbit polyclonal to TNF Receptor I (phospho S274)
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S274 of human TNFR.

  • Positive control
    • Mouse uterus tissue lysate; human hepatocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab60031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

Target

  • Function
    Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
  • Involvement in disease
    Familial hibernian fever
    Multiple sclerosis 5
  • Sequence similarities
    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • Domain
    The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
    Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
  • Post-translational
    modifications
    The soluble form is produced from the membrane form by proteolytic processing.
  • Cellular localization
    Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD120a antibody
    • FPF antibody
    • MGC19588 antibody
    • p55 antibody
    • p55-R antibody
    • p60 antibody
    • TBP1 antibody
    • TBPI antibody
    • TNF R antibody
    • TNF R55 antibody
    • TNF-R1 antibody
    • TNF-RI antibody
    • TNFAR antibody
    • TNFR-I antibody
    • TNFR1 antibody
    • TNFR55 antibody
    • TNFR60 antibody
    • TNFRI antibody
    • TNFRSF1a antibody
    • TNR1A_HUMAN antibody
    • Tumor necrosis factor receptor 1 antibody
    • Tumor necrosis factor receptor superfamily, member 1A antibody
    • Tumor necrosis factor receptor type 1 antibody
    • Tumor necrosis factor receptor type I antibody
    • Tumor necrosis factor-binding protein 1 antibody
    see all

Images

  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab60031 at a dilution of 1/50. A peroxidase-conjugated secondary antibody was used, followed by AEC staining.
  • All lanes : Anti-TNF Receptor I (phospho S274) antibody (ab60031) at 1/100 dilution

    Lane 1 : mouse uterus tissue lysate
    Lane 2 : mouse uterus tissue lysate with excess amount of blocking peptide


    Predicted band size : 51 kDa

References

ab60031 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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