Anti-TNF Receptor II antibody [EPR1653] (ab109322)




Our Abpromise guarantee covers the use of ab109322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 73 kDa (predicted molecular weight: 48 kDa).Can be blocked with TNF Receptor II peptide (ab192471).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
ICC 1/250 - 1/500.

For best results use 100% Methanol fixation.

Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • FunctionReceptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
  • Sequence similaritiesContains 4 TNFR-Cys repeats.
  • Post-translational
    Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
    A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD120b antibody
    • p75 antibody
    • p75 TNF receptor antibody
    • p75TNFR antibody
    • p80 TNF alpha receptor antibody
    • p80 TNF-alpha receptor antibody
    • Soluble TNFR1B variant 1 antibody
    • TBP-2 antibody
    • TBPII antibody
    • TNF R II antibody
    • TNF R2 antibody
    • TNF R75 antibody
    • TNF-R2 antibody
    • TNF-RII antibody
    • TNFBR antibody
    • TNFR-II antibody
    • TNFR1B antibody
    • TNFR2 antibody
    • TNFR80 antibody
    • TNFRII antibody
    • Tnfrsf1b antibody
    • TNR1B_HUMAN antibody
    • Tumor necrosis factor beta receptor antibody
    • Tumor necrosis factor receptor 2 antibody
    • Tumor necrosis factor receptor superfamily member 1B antibody
    • Tumor necrosis factor receptor type II antibody
    • Tumor necrosis factor-binding protein 2 antibody
    see all

Anti-TNF Receptor II antibody [EPR1653] images

  • Overlay histogram showing HL60 cells stained with ab109322 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109322, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-TNF Receptor II antibody [EPR1653] (ab109322) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : SW480 cell lysate
    Lane 4 : THP1 cell lysate

    Lysates/proteins at 10 µg per lane.

    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 48 kDa
    Observed band size : 73 kDa (why is the actual band size different from the predicted?)
  • ab109322 staining TNF Receptor II in SW480 cells. The cells were fixed with 100% methanol. In our hands PFA fixed cells did not produce good results with this antibody. Nuclear DNA was labelled in blue with DAPI.

  • ab109322, at a 1/100 dilution, staining TNF Receptor II in paraffin embedded uterus tissue by Immunohistochemistry.
  • Primary: ab109322, 1/1000 dilution

    Sample: Jurkat whole cell lysate, 100 ug

    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated, 1/1000 dilution

    Capture antibody: 1:40 dilution (1µg in 1 mg lysate)

    Blocking and diluting buffer: 5% NFDM/TBST

References for Anti-TNF Receptor II antibody [EPR1653] (ab109322)

This product has been referenced in:
  • Huang B  et al. Renal Tumor Necrosis Factor a Contributes to Hypertension in Dahl Salt-Sensitive Rats. Sci Rep 6:21960 (2016). IHC ; Rat . Read more (PubMed: 26916681) »
  • Lee SM  et al. Circulating TNF receptors are significant prognostic biomarkers for idiopathic membranous nephropathy. PLoS One 9:e104354 (2014). IHC ; Human . Read more (PubMed: 25098821) »

See all 3 Publications for this product

Product Wall

Thank you very much for your reply and for your call today.

I'm sending a free of charge vial of ab7187 RAW 264.7 cell lysate, on the order ***. This lysate should arrive tomorrow.

Please keep me updated about the results with ab109...

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Thank you again for your email, and for your patience.

I've heard back from the lab, and they load 10 ug of protein per lane. None of the cell lines are transfected, and they use the general Epitomics Western blot protocol-


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Thank you very much for your reply.

I've asked the lab whether the cells are transfected, and how many micrograms of lysate were loaded.

I'd be happy to send a positive control lysate, however I can't send all of the lysates shown i...

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Thank you very much for your call yesterday.

I've received the attached image from the lab, which shows Western blot results using C6, Raw264.7, PC12, and NIH/3T3 cell lysates.

The band probably aligns most closely with the ˜ 60 kDa...

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