The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/5000.
WB: 1/500 - 1/1000. Detects bands of approximately 34, 60 and 68 kDa (predicted molecular weight: 36 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionSubstrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(BACURD2) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the BACURD family. Contains 1 BTB (POZ) domain.
Post-translational modificationsPhosphorylation at Ser-280 by CK2 facilitates the nucleus localization and increases interaction with PCNA.
Cellular localizationCytoplasm. Nucleus. Endosome. Colocalizes with RHOB in endosomes.
BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2 antibody
BTB/POZ domain-containing protein TNFAIP1 antibody
Protein B12 antibody
Tumor necrosis factor alpha induced protein 1 (endothelial) antibody
Tumor necrosis factor alpha induced protein 1 antibody
Tumor necrosis factor antibody
Anti-TNFAIP1 antibody images
Western blot - TNFAIP1 antibody (ab72626)
All lanes : Anti-TNFAIP1 antibody (ab72626) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells, plus PMA (125ng/ml, 30mins) Lane 2 : Extracts from HUVEC cells, plus serum (20%, 30mins) Lane 3 : Extracts from HUVEC cells, plus PMA (125ng/ml, 30mins) and serum (20%, 30mins) plus immunizing peptide