Overview

  • Product name
  • Description
    Rabbit polyclonal to TNFAIP1
  • Specificity
    No cross reactivity with other proteins.
  • Tested applications
    Suitable for: ICC, WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to a sequence at the N-terminal of Human TNFAIP1

  • Positive control
    • Human rectal cancer tissue; rat brain, heart, lung and thymus tissue lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide, 0.01% Thimerosal (merthiolate)
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 0.25 - 0.5 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use a concentration of 0.25 - 0.5 µg/ml.

Target

  • Function
    Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(BACURD2) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the BACURD family.
    Contains 1 BTB (POZ) domain.
  • Post-translational
    modifications
    Phosphorylation at Ser-280 by CK2 facilitates the nucleus localization and increases interaction with PCNA.
  • Cellular localization
    Cytoplasm. Nucleus. Endosome. Colocalizes with RHOB in endosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha-induced protein 1 antibody
    • BACD2_HUMAN antibody
    • BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2 antibody
    • BTB/POZ domain-containing protein TNFAIP1 antibody
    • endothelial antibody
    • hBACURD2 antibody
    • Protein B12 antibody
    • tnfaip1 antibody
    • Tumor necrosis factor alpha induced protein 1 (endothelial) antibody
    • Tumor necrosis factor alpha induced protein 1 antibody
    • Tumor necrosis factor antibody
    see all

Anti-TNFAIP1 antibody images

  • ab86934 at 1µg/ml staining TNFAIP1 in paraffin-embedded human rectal cancer tissue by Immunohistochemistry.
  • All lanes : Anti-TNFAIP1 antibody (ab86934) at 0.5 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat thymus tissue lysate
    Lane 3 : Rat heart tissue lysate
    Lane 4 : Rat lung tissue lysate


    Predicted band size : 36 kDa
    Observed band size : 36 kDa

References for Anti-TNFAIP1 antibody (ab86934)

ab86934 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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