Anti-TNFAIP3 antibody (ab93868)
Key features and details
- Rabbit polyclonal to TNFAIP3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TNFAIP3 antibody
See all TNFAIP3 primary antibodies -
Description
Rabbit polyclonal to TNFAIP3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to a region within amino acids 525-575 (LQDVTRTFNG ICSTCFKRTT AEASSSLSTS LPPSCHQRSK SDPSRLVRSP S) of Human TNFAIP3 (NP_006281.1).
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Positive control
- HeLa whole cell lysate (ab150035)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab93868 was affinity purified using an epitope specific to TNFAIP3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 90 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 90 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. -
Sequence similarities
Belongs to the peptidase C64 family.
Contains 7 A20-type zinc fingers.
Contains 1 OTU domain. -
Domain
The A20-type zinc fingers mediate the ubiquitin ligase activity.
The OTU domain mediates the deubiquitinase activity. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7128 Human
- Omim: 191163 Human
- SwissProt: P21580 Human
- Unigene: 211600 Human
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Alternative names
- A20 antibody
- AISBL antibody
- MGC104522 antibody
see all
Images
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All lanes : Anti-TNFAIP3 antibody (ab93868) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 90 kDa
Exposure time: 3 minutes -
ab93868, at 1 µg/ml, detecting TNFAIP3 in HeLa whole cell lysate by WB of IP.
Lane 1: IP with ab93868 at 3 µg/mg of lysate.
Lane 2: Control IgG.
1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence an with exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab93868 has been referenced in 1 publication.
- Kunzmann S et al. A20 Is Increased in Fetal Lung in a Sheep LPS Model of Chorioamnionitis. Oxid Med Cell Longev 2022:6421419 (2022). PubMed: 35096271