• Product nameAnti-TNFAIP3 antibody
    See all TNFAIP3 primary antibodies
  • Description
    Rabbit polyclonal to TNFAIP3
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 525-575 (LQDVTRTFNG ICSTCFKRTT AEASSSLSTS LPPSCHQRSK SDPSRLVRSP S) of Human TNFAIP3 (NP_006281.1).

  • Positive control
    • HeLa whole cell lysate



Our Abpromise guarantee covers the use of ab93868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIP: Use at 2-5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 90 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionUbiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system.
    • Sequence similaritiesBelongs to the peptidase C64 family.
      Contains 7 A20-type zinc fingers.
      Contains 1 OTU domain.
    • DomainThe A20-type zinc fingers mediate the ubiquitin ligase activity.
      The OTU domain mediates the deubiquitinase activity.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • A20 antibody
      • AISBL antibody
      • MGC104522 antibody
      • MGC138687 antibody
      • MGC138688 antibody
      • OTU domain containing protein 7C antibody
      • OTU domain-containing protein 7C antibody
      • OTUD7C antibody
      • Putative DNA binding protein A20 antibody
      • Putative DNA-binding protein A20 antibody
      • TNAP3_HUMAN antibody
      • TNF alpha-induced protein 3 antibody
      • TNFA1P2 antibody
      • TNFAIP 3 antibody
      • TNFAIP3 (A20) antibody
      • TNFAIP3 antibody
      • Tumor necrosis factor alpha induced protein 3 antibody
      • Tumor necrosis factor alpha-induced protein 3 antibody
      • Tumor necrosis factor induced protein 3 antibody
      • Tumor necrosis factor inducible protein A20 antibody
      • tumor necrosis factor, alpha-induced protein 3 antibody
      • Zinc finger protein A20 antibody
      see all

    Anti-TNFAIP3 antibody images

    • All lanes : Anti-TNFAIP3 antibody (ab93868) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 90 kDa

      Exposure time : 3 minutes
    • ab93868, at 1 µg/ml, detecting TNFAIP3 in HeLa whole cell lysate by WB of IP.
      Lane 1: IP with ab93868 at 3 µg/mg of lysate.
      Lane 2: Control IgG.
      1 mg of lysate was used for IP and 20% of the IP was loaded.
      Detection: Chemiluminescence an with exposure time of 30 seconds.

    References for Anti-TNFAIP3 antibody (ab93868)

    ab93868 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (macrophage)
    Specification macrophage
    Treatment 0.1 ug/ml LPS 24 h
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Dec 12 2014