Overview

  • Product name
  • Description
    Rabbit polyclonal to TNFAIP3
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 525-575 (LQDVTRTFNG ICSTCFKRTT AEASSSLSTS LPPSCHQRSK SDPSRLVRSP S) of Human TNFAIP3 (NP_006281.1).

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab93868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2-5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 90 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system.
    • Sequence similarities
      Belongs to the peptidase C64 family.
      Contains 7 A20-type zinc fingers.
      Contains 1 OTU domain.
    • Domain
      The A20-type zinc fingers mediate the ubiquitin ligase activity.
      The OTU domain mediates the deubiquitinase activity.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • A20 antibody
      • AISBL antibody
      • MGC104522 antibody
      • MGC138687 antibody
      • MGC138688 antibody
      • OTU domain containing protein 7C antibody
      • OTU domain-containing protein 7C antibody
      • OTUD7C antibody
      • Putative DNA binding protein A20 antibody
      • Putative DNA-binding protein A20 antibody
      • TNAP3_HUMAN antibody
      • TNF alpha-induced protein 3 antibody
      • TNFA1P2 antibody
      • TNFAIP 3 antibody
      • TNFAIP3 (A20) antibody
      • TNFAIP3 antibody
      • Tumor necrosis factor alpha induced protein 3 antibody
      • Tumor necrosis factor alpha-induced protein 3 antibody
      • Tumor necrosis factor induced protein 3 antibody
      • Tumor necrosis factor inducible protein A20 antibody
      • tumor necrosis factor, alpha-induced protein 3 antibody
      • Zinc finger protein A20 antibody
      see all

    Images

    • All lanes : Anti-TNFAIP3 antibody (ab93868) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 90 kDa


      Exposure time : 3 minutes
    • ab93868, at 1 µg/ml, detecting TNFAIP3 in HeLa whole cell lysate by WB of IP.
      Lane 1: IP with ab93868 at 3 µg/mg of lysate.
      Lane 2: Control IgG.
      1 mg of lysate was used for IP and 20% of the IP was loaded.
      Detection: Chemiluminescence an with exposure time of 30 seconds.

    References

    ab93868 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (macrophage)
    Specification
    macrophage
    Treatment
    0.1 ug/ml LPS 24 h
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 12 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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