Overview

  • Product nameAnti-TNFAIP8 antibody
    See all TNFAIP8 primary antibodies
  • Description
    Rabbit polyclonal to TNFAIP8
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal sequence of human TNFAIP8.

  • Positive control
    • HUVEC cells. A549 cells. Lung carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab64988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF 1/500 - 1/1000.
ELISA 1/10000.

Target

  • FunctionActs as a negative mediator of apoptosis and may play a role in tumor progression. Suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation.
  • Tissue specificityExpressed at high levels in the spleen, lymph node, thymus, thyroid, bone marrow and placenta. Expressed at high levels both in various tumor tissues, unstimulated and cytokine-activated cultured cells. Expressed at low levels in the spinal cord, ovary, lung, adrenal glands, heart, brain, testis and skeletal muscle.
  • Sequence similaritiesBelongs to the TNFAIP8 family.
  • Developmental stageExpressed at high levels in the fetal liver, lung and kidney.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • GG2 1 antibody
    • Head and neck tumor and metastasis-related protein antibody
    • MDC-3.13 antibody
    • NDED antibody
    • NF-kappa-B-inducible DED-containing protein antibody
    • SCC S2 antibody
    • SCC-S2 antibody
    • SCCS2 antibody
    • TFIP8_HUMAN antibody
    • TNF alpha-induced protein 8 antibody
    • TNF induced protein antibody
    • TNF-induced protein GG2-1 antibody
    • TNFAIP 8 antibody
    • TNFAIP8 antibody
    • Tumor necrosis factor alpha induced protein 8 antibody
    • Tumor necrosis factor alpha-induced protein 8 antibody
    see all

Anti-TNFAIP8 antibody images

  • All lanes : Anti-TNFAIP8 antibody (ab64988) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells
    Lane 2 : Extracts from HUVEC cells with immunizing peptide at 5 µg

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 23 kDa
    Observed band size : 23 kDa
    Additional bands at : 30 kDa,80 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of A549 cells, using TNFAIP8 antibody (ab64988) at 1/500 dilution, in the presence (right panel) and absence (left panel) of immunising peptide.
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using TNFAIP8 antibody (ab64988) at 1/50 dilution, in the presence (right panel) and absence (left panel) of immunising peptide. Antigen retrieval was performed by boiling in a pressure cooker for 2-3 min. A Polymer system was used for signal enhancing. Stained using DAB.

References for Anti-TNFAIP8 antibody (ab64988)

This product has been referenced in:
  • Liu T  et al. TNFAIP8 as a predictor of metastasis and a novel prognostic biomarker in patients with epithelial ovarian cancer. Br J Cancer 109:1685-92 (2013). Read more (PubMed: 23982604) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"