Overview

  • Product name
  • Description
    Rabbit polyclonal to TNIK
  • Specificity
    BLAST analysis of the peptide immunogen showed no homology with other human proteins.
  • Tested applications
    Suitable for: IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Hamster, Cow, Dog, Xenopus laevis, Lizard, Monkey, Gorilla, Opossum, Common marmoset, Bat, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human TNIK (internal sequence). Synthetic peptide corresponding to 18 amino acids within TNIK (Q9UKE5, NM_015028, BAA25477.2).

  • Positive control
    • Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab150739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.

Target

  • Function
    Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading.
  • Tissue specificity
    Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CNH domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated. Autophosphorylation is activated by RAP2A and induces association to the cytoskeletal fraction.
  • Cellular localization
    Nucleus. Cytoplasm. Recycling endosome. Cytoplasm > cytoskeleton. Associated with recycling endosomes and the cytoskeletal fraction upon RAP2A overexpression.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.7.11.1 antibody
    • KIAA0551 antibody
    • Tnik antibody
    • TNIK_HUMAN antibody
    • Traf2 and NCK interacting kinase antibody
    • Traf2 and NCK-interacting protein kinase antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labelled with ab150739 at 2 µg/ml.

References

ab150739 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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