• Product name
  • Description
    Rabbit polyclonal to TNIK
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 600 and 650 of Human TNIK (NP_055843.1).

  • Positive control
    • HeLa whole cell lysate.



Our Abpromise guarantee covers the use of ab95887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 155 kDa.
IP Use at 2-5 µg/mg of lysate.


  • Function
    Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading.
  • Tissue specificity
    Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CNH domain.
    Contains 1 protein kinase domain.
  • Post-translational
    Autophosphorylated. Autophosphorylation is activated by RAP2A and induces association to the cytoskeletal fraction.
  • Cellular localization
    Nucleus. Cytoplasm. Recycling endosome. Cytoplasm > cytoskeleton. Associated with recycling endosomes and the cytoskeletal fraction upon RAP2A overexpression.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC antibody
    • KIAA0551 antibody
    • Tnik antibody
    • TNIK_HUMAN antibody
    • Traf2 and NCK interacting kinase antibody
    • Traf2 and NCK-interacting protein kinase antibody
    see all

Anti-TNIK antibody images

  • All lanes : Anti-TNIK antibody (ab95887) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Predicted band size : 155 kDa

    Exposure time : 3 minutes
  • Detection of TNIK by Western Blot of Immunprecipitate.
    ab95887 at 1µg/ml staining TNIK in HeLa whole cell lysate immunoprecipitated using ab95887 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References for Anti-TNIK antibody (ab95887)

ab95887 has not yet been referenced specifically in any publications.

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