The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Application notesIs unsuitable for WB.
FunctionSerine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading.
Tissue specificityExpressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CNH domain. Contains 1 protein kinase domain.
Post-translational modificationsAutophosphorylated. Autophosphorylation is activated by RAP2A and induces association to the cytoskeletal fraction.
Cellular localizationNucleus. Cytoplasm. Recycling endosome. Cytoplasm > cytoskeleton. Associated with recycling endosomes and the cytoskeletal fraction upon RAP2A overexpression.
Detection of TNIK by Western Blot of Immunprecipitate.
anti-TNIK at 1µg/ml staining TNIK in HeLa whole cell lysate immunoprecipitated using ab95888 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
References for Anti-TNIK antibody (ab95888)
has not yet been referenced specifically in any publications.
Publishing research using ab95888? Please let us know so that we can cite the reference in this datasheet.
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