Overview

  • Product nameAnti-TNIP1 antibody
    See all TNIP1 primary antibodies
  • Description
    Mouse polyclonal to TNIP1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    TNIP1 full-length human protein (NP_006049)

  • Positive control
    • TNIP1 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab70152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 72 kDa).

Target

  • FunctionInteracts with zinc finger protein A20/TNFAIP3 and inhibits TNF-induced NF-kappa-B-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal (By similarity). Increases cell surface CD4(T4) antigen expression. Interacts with HIV-1 matrix protein and is packaged into virions and overexpression can inhibit viral replication. May regulate matrix nuclear localization, both nuclear import of PIC (Preintegration complex) and export of GAG polyprotein and viral genomic RNA during virion production.
  • Tissue specificityUbiquitous. Strongly expressed in peripheral blood lymphocytes, spleen and skeletal muscle, and is weakly expressed in the brain.
  • Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABIN 1 antibody
    • HIV-1 Nef-interacting protein antibody
    • hVAN antibody
    • KIAA0113 antibody
    • Naf1 antibody
    • Nef-associated factor 1 antibody
    • Nip40-1 antibody
    • TNFAIP3 interacting protein 1 antibody
    • TNFAIP3-interacting protein 1 antibody
    • TNIP-1 antibody
    • Tnip1 antibody
    • TNIP1_HUMAN antibody
    • VAN antibody
    • Virion-associated nuclear shuttling protein antibody
    see all

Anti-TNIP1 antibody images

  • All lanes : Anti-TNIP1 antibody (ab70152) at 1/500 dilution

    Lane 1 : TNIP1 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 72 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

References for Anti-TNIP1 antibody (ab70152)

ab70152 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (macrophage)
Specification macrophage
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 12 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"