Overview

  • Product name
  • Description
    Mouse polyclonal to TNIP1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    TNIP1 full-length human protein (NP_006049)

  • Positive control
    • TNIP1 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab70152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 72 kDa).

Target

  • Function
    Interacts with zinc finger protein A20/TNFAIP3 and inhibits TNF-induced NF-kappa-B-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal (By similarity). Increases cell surface CD4(T4) antigen expression. Interacts with HIV-1 matrix protein and is packaged into virions and overexpression can inhibit viral replication. May regulate matrix nuclear localization, both nuclear import of PIC (Preintegration complex) and export of GAG polyprotein and viral genomic RNA during virion production.
  • Tissue specificity
    Ubiquitous. Strongly expressed in peripheral blood lymphocytes, spleen and skeletal muscle, and is weakly expressed in the brain.
  • Cellular localization
    Cytoplasm. Nucleus. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABIN 1 antibody
    • HIV-1 Nef-interacting protein antibody
    • hVAN antibody
    • KIAA0113 antibody
    • Naf1 antibody
    • Nef-associated factor 1 antibody
    • Nip40-1 antibody
    • TNFAIP3 interacting protein 1 antibody
    • TNFAIP3-interacting protein 1 antibody
    • TNIP-1 antibody
    • Tnip1 antibody
    • TNIP1_HUMAN antibody
    • VAN antibody
    • Virion-associated nuclear shuttling protein antibody
    see all

Images

  • All lanes : Anti-TNIP1 antibody (ab70152) at 1/500 dilution

    Lane 1 : TNIP1 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 72 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

References

ab70152 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (macrophage)
Specification
macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Submitted Dec 12 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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