Anti-TNIP2 antibody (ab155513)
Key features and details
- Rabbit polyclonal to TNIP2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TNIP2 antibody
See all TNIP2 primary antibodies -
Description
Rabbit polyclonal to TNIP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 46-308 of Human TNIP2 (UniProt ID: Q8NFZ5).
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji whole cell lysate; HeLa cells; Human SAS xenograft tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 49 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 49 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Inhibits NF-kappa-B activation by blocking the interaction of RIPK1 with its downstream effector IKBKG. Forms a ternary complex with NFKB1 and MAP3K8 but appears to function upstream of MAP3K8 in the TLR4 signaling pathway that regulates MAP3K8 activation. -
Tissue specificity
Ubiquitously expressed in all tissues examined. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 79155 Human
- Omim: 610669 Human
- SwissProt: Q8NFZ5 Human
- Unigene: 726088 Human
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Alternative names
- A20 binding inhibitor of NF kappaB activation 2 antibody
- A20-binding inhibitor of NF-kappa-B activation 2 antibody
- ABIN 2 antibody
see all
Images
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All lanes : Anti-TNIP2 antibody (ab155513) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLaS3 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 49 kDa
10% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling TNIP2 at 1/200 dilution. Lower lower image merged with DNA probe.
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Immunohistochemical analysis of paraffin embedded Human SAS xenograft tissue labeling TNIP2 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155513 has not yet been referenced specifically in any publications.