Overview

  • Product name
  • Description
    Rabbit polyclonal to TNIP2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 46-308 of Human TNIP2 (UniProt ID: Q8NFZ5).

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji whole cell lysate; HeLa cells; Human SAS xenograft tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab155513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 49 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Inhibits NF-kappa-B activation by blocking the interaction of RIPK1 with its downstream effector IKBKG. Forms a ternary complex with NFKB1 and MAP3K8 but appears to function upstream of MAP3K8 in the TLR4 signaling pathway that regulates MAP3K8 activation.
  • Tissue specificity
    Ubiquitously expressed in all tissues examined.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • A20 binding inhibitor of NF kappaB activation 2 antibody
    • A20-binding inhibitor of NF-kappa-B activation 2 antibody
    • ABIN 2 antibody
    • ABIN-2 antibody
    • Fetal liver LKB1-interacting protein antibody
    • FLIP1 antibody
    • KLIP antibody
    • LKB1 interacting protein antibody
    • TNFAIP3 interacting protein 2 antibody
    • TNFAIP3-interacting protein 2 antibody
    • TNIP2 antibody
    • TNIP2_HUMAN antibody
    see all

Images

  • All lanes : Anti-TNIP2 antibody (ab155513) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : HeLaS3 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 49 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling TNIP2 at 1/200 dilution. Lower lower image merged with DNA probe.
  • Immunohistochemical analysis of paraffin embedded Human SAS xenograft tissue labeling TNIP2 at 1/500 dilution.

References

ab155513 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (macrophage)
Specification
macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 12 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up