Overview

  • Product nameAnti-Tnk1 antibody [3F11H6]
    See all Tnk1 primary antibodies
  • Description
    Mouse monoclonal [3F11H6] to Tnk1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified truncated recombinant human Tnk1 (aa451-560)-His expressed in E.coli strain BL21 (DE3)

  • Positive control
    • Truncated Tnk1-His recombinant protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab70402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: 1/10000.
    WB: 1/500 - 1/2000. Detects a band of approximately 17 kDa (predicted molecular weight: 73 kDa). Note: ab70402 has only been tested against a truncated recombinant protein.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in negative regulation of cell growth. Has tumor suppressor properties. Plays a negative regulatory role in the Ras-MAPK pathway. May function in signaling pathways utilized broadly during fetal development and more selectively in adult tissues and in cells of the lymphohematopoietic system. Could specifically be involved in phospholipid signal transduction.
    • Tissue specificityExpressed in all umbilical cord blood, bone marrow and adult blood cell sub-populations and in several leukemia cell lines. Highly expressed in fetal blood, brain, lung, liver and kidney. Detected at lower levels in adult prostate, testis, ovary, small intestine and colon. Not expressed in adult lung, liver, kidney or brain.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
      Contains 1 protein kinase domain.
      Contains 1 SH3 domain.
    • Post-translational
      modifications
      Autophosphorylated on tyrosine residues.
    • Cellular localizationCytoplasm. Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD38 negative kinase 1 antibody
      • EC 2.7.10.2 antibody
      • Kinase of embryonic stem cells antibody
      • Kos 1 antibody
      • Kos1 antibody
      • MGC46193 antibody
      • Non receptor tyrosine protein kinase TNK 1 antibody
      • Non receptor tyrosine protein kinase TNK1 antibody
      • Non-receptor tyrosine-protein kinase TNK1 antibody
      • Tnk 1 antibody
      • Tnk1 antibody
      • TNK1_HUMAN antibody
      • Tyrosine kinase non receptor 1 antibody
      see all

    Anti-Tnk1 antibody [3F11H6] images

    • All lanes : Anti-Tnk1 antibody [3F11H6] (ab70402) at 1/500 dilution

      Lane 1 : Molecular weight markers
      Lane 2 : Truncated Tnk1-His recombinant protein


      Predicted band size : 73 kDa
      Observed band size : 17 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-Tnk1 antibody [3F11H6] (ab70402)

    ab70402 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab70402.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"