Overview

  • Product nameAnti-TNNI1 antibody [12F10]
    See all TNNI1 primary antibodies
  • Description
    Mouse monoclonal [12F10] to TNNI1
  • SpecificityDetects only slow Troponin I from human skeletal muscle. Cross-reaction of this antibody with cardiac troponin I is less then 0.05%.
  • Tested applicationsSuitable for: Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS with 0.1% sodium azide, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number12F10
  • MyelomaSp2/0
  • IsotypeIgG2b
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [7G2] to Troponin I fast + slow skeletal muscle (HRP) (ab24464). Use as Capture Antibody.
AP Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

Target

  • FunctionTroponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Tissue specificityHighest levels observed in human skeletal muscle (e.g. gastrocnemious muscle), differentiated cultures of primary human muscle cells and rhabdomyosarcoma cells cultured in low serum medium. Expressed in C2 muscle cell myoblasts and myotubes.
  • Sequence similaritiesBelongs to the troponin I family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2700018B22Rik antibody
    • AI747285 antibody
    • DKFZp451O223 antibody
    • si:dz182n13.3 antibody
    • slow skeletal muscle antibody
    • slow-twitch isoform antibody
    • SSTNI antibody
    • TNN1 antibody
    • Tnni1 antibody
    • TNNI1_HUMAN antibody
    • Troponin I antibody
    • Troponin I slow skeletal muscle antibody
    • Troponin I slow twitch isoform antibody
    • troponin I type 1 (skeletal, slow) antibody
    • Troponin I, slow isoform antibody
    see all

Anti-TNNI1 antibody [12F10] images

  • All lanes : Anti-TNNI1 antibody [12F10] (ab8293) at 1 µg/ml

    Lane 1 : 40ug human muscle lysate (whole tissue)
    Lane 2 : 40ug human muscle lysate (whole tissue)
    Lane 3 : 40ug human muscle lysate (whole tissue)
    Lane 4 : 40ug human muscle lysate (whole tissue)
    Lane 5 : 40ug human muscle lysate (whole tissue)
    Lane 6 : 40ug human muscle lysate (whole tissue)
    Lane 7 : 40ug rat adipose lysate (whole tissue)

    Secondary
    HRP donkey anti-mouse HRP
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Observed band size : 22 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-TNNI1 antibody [12F10] (ab8293)

This product has been referenced in:
  • Zhou Y  et al. Pitx2 regulates myosin heavy chain isoform expression and multi-innervation in extraocular muscle. J Physiol 589:4601-14 (2011). Read more (PubMed: 21727215) »
  • Groshong JS  et al. Calpain activation impairs neuromuscular transmission in a mouse model of the slow-channel myasthenic syndrome. J Clin Invest 117:2903-12 (2007). WB ; Human . Read more (PubMed: 17853947) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (skeletal muscle)
Loading amount 30 µg
Specification skeletal muscle
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Aug 19 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Muscle biopsy material)
Loading amount 40 µg
Specification Muscle biopsy material
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5%
Username

Abcam user community

Verified customer

Submitted Oct 26 2006

Thank you for your enquiry. Unfortunately we have not determined the epitopes. However, the antibodies are forming a pair in ELISA. Recommended pair is 12F10 - 7G2 (capture - conjugate).

Unfortunately, we do not sell ab8294 conjugated to HRP. You will need to conjugate this antibody to HRP yourself in order to use it in a sandwich ELISA. ab9920 (human skeletal muscle Troponin I) should be suitable for use as a positive control (we can...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"