Overview

  • Product name
  • Description
    Rabbit polyclonal to TNNI1
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A peptide from near the N terminal residues of human TNNI1 protein (NM_003281).

  • Positive control
    • Skeletal muscle

Properties

  • Form
    Lyophilised:Reconstitute in 200 µl sterile H2O.
  • Storage instructions
    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 2% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified by peptide affinity column.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
  • Application notes
    ELISA: Use at an assay dependent dilution.
    Peptide ELISA titer, 1:1,000-1:10,000.
    IHC-P: 1/100 - 1/500.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
    • Tissue specificity
      Highest levels observed in human skeletal muscle (e.g. gastrocnemious muscle), differentiated cultures of primary human muscle cells and rhabdomyosarcoma cells cultured in low serum medium. Expressed in C2 muscle cell myoblasts and myotubes.
    • Sequence similarities
      Belongs to the troponin I family.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2700018B22Rik antibody
      • AI747285 antibody
      • DKFZp451O223 antibody
      • si:dz182n13.3 antibody
      • slow skeletal muscle antibody
      • slow-twitch isoform antibody
      • SSTNI antibody
      • TNN1 antibody
      • Tnni1 antibody
      • TNNI1_HUMAN antibody
      • Troponin I antibody
      • Troponin I slow skeletal muscle antibody
      • Troponin I slow twitch isoform antibody
      • troponin I type 1 (skeletal, slow) antibody
      • Troponin I, slow isoform antibody
      see all

    Images

    • Immunohistochemical analysis of TNNI1 expression in formalin fixed, paraffin embedded skeletal muscle using ab85087 at a 1/100 dilution. Note cytoplasmic staining.

    References

    This product has been referenced in:
    • Grajales L  et al. Induction of cardiac myogenic lineage development differs between mesenchymal and satellite cells and is accelerated by bone morphogenetic protein-4. J Mol Cell Cardiol 53:382-91 (2012). ICC/IF ; Human . Read more (PubMed: 22709559) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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