Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Highest levels observed in human skeletal muscle (e.g. gastrocnemious muscle), differentiated cultures of primary human muscle cells and rhabdomyosarcoma cells cultured in low serum medium. Expressed in C2 muscle cell myoblasts and myotubes.
Western blot - Anti-TNNI1 [AT36E7] antibody (ab171650)
Lane 1 : Anti-TNNI1 antibody [AT36E7] (ab171650) at 1/500 dilution Lane 2 : Anti-TNNI1 antibody [AT36E7] (ab171650) at 1/1000 dilution Lane 3 : Anti-TNNI1 antibody [AT36E7] (ab171650) at 1/5000 dilution
Lane 1 : Mouse muscle lysate Lane 2 : Mouse muscle lysate Lane 3 : Mouse muscle lysate
Lysates/proteins at 40 µg per lane.
Secondary Goat anti-mouse conjugated to HRP Developed using the ECL technique