Anti-TNNT3 antibody - C-terminal (ab175058)
Key features and details
- Goat polyclonal to TNNT3 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TNNT3 antibody - C-terminal
See all TNNT3 primary antibodies -
Description
Goat polyclonal to TNNT3 - C-terminal -
Host species
Goat -
Specificity
ab175058 is expected to recognize isoform 1, 2 and 4 (NP_006748.1; NP_001036246.1; NP_001036247.1). -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human TNNT3 aa 240-254 (C terminal) (Cysteine residue). The exact sequence is proprietary. NP_006748.1; NP_001036246.1; NP_001036247.1
Sequence:TTLRSRIDQAQKHSK
Database link: P45378 -
Positive control
- Human, Mouse and Rat skeletal muscle lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab175058 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (1) |
Use a concentration of 4 - 8 µg/ml.
|
WB |
Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 32 kDa.
1 hour primary incubation is recommended for this product. |
Notes |
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IHC-P
Use a concentration of 4 - 8 µg/ml. |
WB
Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 32 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Function
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. -
Tissue specificity
In fetal and adult fast skeletal muscles, with a higher level expression in fetal than in adult muscle. -
Involvement in disease
Defects in TNNT3 are a cause of distal arthrogryposis type 2B (DA2B) [MIM:601680]; also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. -
Sequence similarities
Belongs to the troponin T family. - Information by UniProt
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Database links
- Entrez Gene: 7140 Human
- Entrez Gene: 21957 Mouse
- Entrez Gene: 24838 Rat
- Omim: 600692 Human
- SwissProt: P45378 Human
- SwissProt: Q9QZ47 Mouse
- SwissProt: P09739 Rat
- Unigene: 73454 Human
see all -
Alternative names
- AMCD2B antibody
- Beta TnTF antibody
- Beta-TnTF antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab175058 has been referenced in 1 publication.
- Lu J et al. The distal arthrogryposis-linked p.R63C variant promotes the stability and nuclear accumulation of TNNT3. J Clin Lab Anal 35:e24089 (2021). PubMed: 34766372