The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 4 - 8 µg/ml.
Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 32 kDa.
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
In fetal and adult fast skeletal muscles, with a higher level expression in fetal than in adult muscle.
Involvement in disease
Defects in TNNT3 are a cause of distal arthrogryposis type 2B (DA2B) [MIM:601680]; also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle labelling TNNT3 with ab175058 at 5µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western blot - Anti-TNNT3 antibody (ab175058)
All lanes : Anti-TNNT3 antibody - C-terminal (ab175058) at 0.01 µg/ml
Lane 1 : Human skeletal muscle lysate Lane 2 : Mouse skeletal muscle lysate Lane 3 : Rat skeletal muscle lysate