Anti-TNNT3 antibody - C-terminal (ab175058)

Overview

  • Product name
    Anti-TNNT3 antibody - C-terminal
    See all TNNT3 primary antibodies
  • Description
    Goat polyclonal to TNNT3 - C-terminal
  • Host species
    Goat
  • Specificity
    ab175058 is expected to recognize isoform 1, 2 and 4 (NP_006748.1; NP_001036246.1; NP_001036247.1).
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TNNT3 aa 240-254 (C terminal) (Cysteine residue). The exact sequence is proprietary. NP_006748.1; NP_001036246.1; NP_001036247.1
    Sequence:

    TTLRSRIDQAQKHSK


    Database link: P45378

  • Positive control
    • Human, Mouse and Rat skeletal muscle lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab175058 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 32 kDa.

Target

  • Function
    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Tissue specificity
    In fetal and adult fast skeletal muscles, with a higher level expression in fetal than in adult muscle.
  • Involvement in disease
    Defects in TNNT3 are a cause of distal arthrogryposis type 2B (DA2B) [MIM:601680]; also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin.
  • Sequence similarities
    Belongs to the troponin T family.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMCD2B antibody
    • Beta TnTF antibody
    • Beta-TnTF antibody
    • DA2B antibody
    • DKFZp779M2348 antibody
    • fast skeletal muscle antibody
    • Fast skeletal muscle troponin T antibody
    • FSSV antibody
    • fTnT antibody
    • TNNT 3 antibody
    • Tnnt3 antibody
    • TNNT3_HUMAN antibody
    • TnTf antibody
    • Troponin T antibody
    • Troponin T fast skeletal muscle antibody
    • Troponin T type 3 (skeletal fast) antibody
    • Troponin T type 3 antibody
    • Troponin T3 skeletal fast antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle labelling TNNT3 with ab175058 at 5µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

  • All lanes : Anti-TNNT3 antibody - C-terminal (ab175058) at 0.01 µg/ml

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Mouse skeletal muscle lysate
    Lane 3 : Rat skeletal muscle lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 32 kDa

References

ab175058 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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