Anti-TNPO3 antibody [3152C2a] (ab54353)
Key features and details
- Mouse monoclonal [3152C2a] to TNPO3
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG2b
Overview
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Product name
Anti-TNPO3 antibody [3152C2a]
See all TNPO3 primary antibodies -
Description
Mouse monoclonal [3152C2a] to TNPO3 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment (Human) from near the N terminus.
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Positive control
- HeLa whole cell lysate (ab150035); NIH3T3 whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
Clonality
Monoclonal -
Clone number
3152C2a -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab54353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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WB | (3) |
1/50 - 1/100. Detects a band of approximately 90 kDa (predicted molecular weight: 110 kDa).
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
WB
1/50 - 1/100. Detects a band of approximately 90 kDa (predicted molecular weight: 110 kDa). |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Seems to function in nuclear protein import as nuclear transport receptor. In vitro, mediates the nuclear import of splicing factor SR proteins SFRS1 and SFRS2, by recognizing phosphorylated RS domains. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 23534 Human
- Entrez Gene: 320938 Mouse
- Omim: 610032 Human
- SwissProt: Q9Y5L0 Human
- SwissProt: Q6P2B1 Mouse
- Unigene: 193613 Human
- Unigene: 276381 Mouse
- Unigene: 304472 Mouse
see all -
Alternative names
- TNPO 3 antibody
- Imp12 antibody
- Importin 12 antibody
see all
Images
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Anti-TNPO3 antibody [3152C2a] (ab54353) at 1/100 dilution + HeLa whole cell lysate at 25 µg
Secondary
anti Mouse IgG at 1/2500 dilution
Predicted band size: 110 kDa
Observed band size: ~90 kDa why is the actual band size different from the predicted? -
Anti-TNPO3 antibody [3152C2a] (ab54353) at 1/50 dilution + NIH3T3 whole cell lysate at 25 µg
Secondary
anti Mouse IgG at 1/2500 dilution
Predicted band size: 110 kDa
Observed band size: ~90 kDa why is the actual band size different from the predicted? -
IHC image of ab54353 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab54353, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Overlay histogram showing HeLa cells stained with ab54353 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54353, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.
Protocols
Datasheets and documents
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Datasheet download
References (20)
ab54353 has been referenced in 20 publications.
- Baade I et al. The RNA-binding protein FUS is chaperoned and imported into the nucleus by a network of import receptors. J Biol Chem 296:100659 (2021). PubMed: 33857479
- Miles RJ et al. MxB sensitivity of HIV-1 is determined by a highly variable and dynamic capsid surface. Elife 9:N/A (2020). PubMed: 32553106
- Gordon DE et al. A Quantitative Genetic Interaction Map of HIV Infection. Mol Cell 78:197-209.e7 (2020). PubMed: 32084337
- Kane M et al. Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2. Elife 7:N/A (2018). PubMed: 30084827
- Jovicic A et al. Modifiers of C9orf72 dipeptide repeat toxicity connect nucleocytoplasmic transport defects to FTD/ALS. Nat Neurosci 18:1226-9 (2015). PubMed: 26308983