• Product nameAnti-TNPO3 antibody
    See all TNPO3 primary antibodies
  • Description
    Rabbit polyclonal to TNPO3
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human, Bird
    Predicted to work with: Cow, Xenopus laevis, Non human primates
  • Immunogen

    Synthetic peptide derived from within residues 100 - 200 of Human TNPO3.

    (Peptide available as ab71387.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat (Staurosporine Treated for 24hr, 500nM), Y79, Ramos, MEF1



Our Abpromise guarantee covers the use of ab71388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 104 kDa).
ICC/IF Use a concentration of 5 µg/ml.


Anti-TNPO3 antibody images

  • All lanes : Anti-TNPO3 antibody (ab71388) at 1 µg/ml

    Lane 1 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
    Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 4 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 104 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab71388 stained MCF7 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71388, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% paraformaldehyde fixed (10 min) HeLa, Hek293 and HepG2 cells at 5µg/ml.

References for Anti-TNPO3 antibody (ab71388)

This product has been referenced in:
  • De Iaco A & Luban J Inhibition of HIV-1 infection by TNPO3 depletion is determined by capsid and detectable after viral cDNA enters the nucleus. Retrovirology 8:98 (2011). WB . Read more (PubMed: 22145813) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Quail Cell lysate - whole cell (fibroblast)
Loading amount 10 µg
Specification fibroblast
Gel Running Conditions Reduced Denaturing (10% Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Nov 16 2012

Expiration date: March 8th, 2012
Value: ***

I am very pleased to hear you would like to accept our offer and test ab71388 in quail. This code will give you *** off your next order before the expiration date. To red...

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