Overview

  • Product name
  • Description
    Rabbit polyclonal to TNPO3
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human, Bird
    Predicted to work with: Cow, Xenopus laevis, Non human primates
  • Immunogen

    Synthetic peptide derived from within residues 100 - 200 of Human TNPO3.

    (Peptide available as ab71387.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat (Staurosporine Treated for 24hr, 500nM), Y79, Ramos, MEF1

Properties

Applications

Our Abpromise guarantee covers the use of ab71388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 104 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

Images

  • All lanes : Anti-TNPO3 antibody (ab71388) at 1 µg/ml

    Lane 1 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
    Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 4 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 104 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab71388 stained MCF7 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71388, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% paraformaldehyde fixed (10 min) HeLa, Hek293 and HepG2 cells at 5µg/ml.

References

This product has been referenced in:
  • De Iaco A  et al. TNPO3 protects HIV-1 replication from CPSF6-mediated capsid stabilization in the host cell cytoplasm. Retrovirology 10:20 (2013). WB . Read more (PubMed: 23414560) »
  • De Iaco A & Luban J Inhibition of HIV-1 infection by TNPO3 depletion is determined by capsid and detectable after viral cDNA enters the nucleus. Retrovirology 8:98 (2011). WB . Read more (PubMed: 22145813) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Quail Cell lysate - whole cell (fibroblast)
Loading amount
10 µg
Specification
fibroblast
Gel Running Conditions
Reduced Denaturing (10% Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Nov 16 2012

DISCOUNT CODE: ***
Expiration date: March 8th, 2012
Value: ***

I am very pleased to hear you would like to accept our offer and test ab71388 in quail. This code will give you *** off your next order before the expiration date. To red...

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