Overview

  • Product nameAnti-TNPO3 antibody [EPR5264]
    See all TNPO3 primary antibodies
  • Description
    Rabbit monoclonal [EPR5264] to TNPO3
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C terminal of Human TNPO3.

  • Positive control
    • HeLa, Jurkat, Ramos and HepG2 cell lysates; Human kidney tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 104 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    Anti-TNPO3 antibody [EPR5264] images

    • All lanes : Anti-TNPO3 antibody [EPR5264] (ab109386) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Ramos cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 104 kDa
    • ab109386, at 1/100 dilution, staining TNPO3 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-TNPO3 antibody [EPR5264] (ab109386)

    This product has been referenced in:
    • Meehan AM  et al. A cyclophilin homology domain-independent role for Nup358 in HIV-1 infection. PLoS Pathog 10:e1003969 (2014). WB . Read more (PubMed: 24586169) »
    • Torella A  et al. Next-generation sequencing identifies transportin 3 as the causative gene for LGMD1F. PLoS One 8:e63536 (2013). WB ; Human . Read more (PubMed: 23667635) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (macrophage)
    Specification macrophage
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Dec 12 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"