Overview

  • Product nameAnti-TNR antibody
    See all TNR primary antibodies
  • Description
    Rabbit polyclonal to TNR
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 748-849 of Human TNR isoform 1 (Q92752).

  • Positive control
    • Human rectum tissue. U-251MG cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab121921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

Target

  • FunctionNeural extracellular matrix (ECM) protein involved in interactions with different cells and matrix components. These interactions can influence cellular behavior by either evoking a stable adhesion and differentiation, or repulsion and inhibition of neurite growth. Binding to cell surface gangliosides inhibits RGD-dependent integrin-mediated cell adhesion and results in an inhibition of PTK2 (FAK) phosphorylation and cell detachment. Binding to membrane surface sulfatides results in a oligodendrocyte adhesion and differentiation. Interaction with CNTN1 induces a repulsion of neurons and an inhibition of neurite outgrowth. Interacts with SCN2B may play a crucial role in clustering and regulation of activity of sodium channels at nodes of Ranvier. TNR-linked chondroitin sulfate glycosaminoglycans are involved in the interaction with FN1 and mediate inhibition of cell adhesion and neurite outgrowth. The highly regulated addition of sulfated carbohydrate structure may modulate the adhesive properties of TNR over the course of development and during synapse maintenance.
  • Tissue specificityBrain specific.
  • Sequence similaritiesBelongs to the tenascin family.
    Contains 5 EGF-like domains.
    Contains 1 fibrinogen C-terminal domain.
    Contains 9 fibronectin type-III domains.
  • DomainThe EGF-like domains mediate interaction with CNTN1. The fibronectin type-III domains 3-5 mediate interaction with BCAN. The fibronectin type-III domains 1-2 and 7-9 mediate interaction with SCN2B.
  • Post-translational
    modifications
    Contains N-linked oligosaccharides, O-linked sialylated structures and O-linked chondroitin sulfate glycosaminoglycans. Contains N-linked oligosaccharides with a sulfated carbohydrate structure (By similarity). O-glycosylated on Thr-36 or Thr-37 with a core 1 or possibly core 8 glycan.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Janusin antibody
    • MGC149328 antibody
    • Restrictin antibody
    • Tenascin R (restrictin, janusin) antibody
    • Tenascin-R antibody
    • TENR_HUMAN antibody
    • TN-R antibody
    • Tnr antibody
    see all

Anti-TNR antibody images

  • ab121921 at 1/50 dilution staining TNR in paraffin-embedded Human rectum tissue by Immunohistochemistry.
  • ab121921 at 4 µg/ml staining TNR in Human cell line U-251MG by Immunofluorescence (green).

References for Anti-TNR antibody (ab121921)

ab121921 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"