Overview

  • Product nameAnti-TNRC6B antibody
    See all TNRC6B primary antibodies
  • Description
    Mouse monoclonal to TNRC6B
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Dog, Rhesus monkey
  • Immunogen

    Recombinant fragment: ECNLGVWKSD PKAKSVQSSN STTENNNGLG NWRNVSGQDR IGPGSGFSNF NPNSNPSAWP ALVQEGTSRK GALETDNSNS SAQVSTVGQT SREQQSKMEN, corresponding to amino acids 253-352 of Human TNRC6B (NP_055903) with tag.

  • Positive control
    • Recombinant tagged human TNRC6B fragment.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
ICC/IF
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 183 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceTNRC6B belongs to the GW182 family. It contains one RRM (RNA recognition motif) domain. TNRC6B plays a role in RNA mediated gene silencing by both micro RNAs (miRNAs) and short interfering RNAs (siRNAs). It is required for miRNA dependent translational repression and siRNA dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. There are three named isoforms.
    • Cellular localizationCytoplasm, P-body. Mammalian P-bodies are also known as GW bodies, GWBs.
    • Database links
    • Alternative names
      • KIAA1093 antibody
      • TNRC 6B antibody
      • Trinucleotide repeat containing 6B antibody
      • Trinucleotide repeat containing gene 6B protein antibody

    Anti-TNRC6B antibody images

    • Anti-TNRC6B antibody (ab77218) at 1 µg/ml + Recombinant tagged human TNRC6B fragment at 0.1 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

      Predicted band size : 183 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescence analysis of HeLa cells, staining TNRC6B with ab77218.

      Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/500 in diluent) for 1 hour at 23°C. An FITC-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody.

      See Abreview

    References for Anti-TNRC6B antibody (ab77218)

    ab77218 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5 % Triton x100
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
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    Verified customer

    Submitted Oct 31 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"