The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 109 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceTNRC6B belongs to the GW182 family. It contains one RRM (RNA recognition motif) domain. TNRC6B plays a role in RNA mediated gene silencing by both micro RNAs (miRNAs) and short interfering RNAs (siRNAs). It is required for miRNA dependent translational repression and siRNA dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. There are three named isoforms.
Cellular localizationCytoplasm, P-body. Mammalian P-bodies are also known as GW bodies, GWBs.