Anti-TNRC6C antibody (ab168838)

Overview

  • Product name
  • Description
    Rabbit polyclonal to TNRC6C
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 550-600 of Human TNRC6C (NP_061869.2).

  • Positive control
    • Jurkat and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab168838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 176 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-TNRC6C antibody (ab168838) at 0.4 µg/ml

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 176 kDa


    Exposure time : 30 seconds
  • Detection of TNRC6C in Immunoprecipitates of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168838 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab168838 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Although we do not have experimental data from knockouts or tests against recombinant protein, alignment of the amino acid sequence that was used to generate the Tnrc6C antibody ab168838 with the amino acid sequences of human Tnrc6a and Tnrc6b suggest ...

Read More
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (4%-12% gel)
Sample
Mouse Cell lysate - whole cell (MEFs)
Specification
MEFs
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 28 2014

Abreviews
Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (7% Novex- homemade)
Sample
Mouse Cell lysate - whole cell (ES cells, NIH-3T3 cells, HEK-293 cells)
Specification
ES cells, NIH-3T3 cells, HEK-293 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Hristo Houbaviy

Verified customer

Submitted Nov 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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