Anti-TNRC6C antibody (ab168838)

Overview

  • Product name
  • Description
    Rabbit polyclonal to TNRC6C
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 550-600 of Human TNRC6C (NP_061869.2).

  • Positive control
    • Jurkat and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab168838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 176 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-TNRC6C antibody (ab168838) at 0.4 µg/ml

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 176 kDa


    Exposure time: 30 seconds
  • Detection of TNRC6C in Immunoprecipitates of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168838 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab168838 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (4%-12% gel)
Sample
Mouse Cell lysate - whole cell (MEFs)
Specification
MEFs
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 28 2014

Abreviews
Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (7% Novex- homemade)
Sample
Mouse Cell lysate - whole cell (ES cells, NIH-3T3 cells, HEK-293 cells)
Specification
ES cells, NIH-3T3 cells, HEK-293 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Hristo Houbaviy

Verified customer

Submitted Nov 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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