Overview

  • Product nameAnti-TNS4 antibody
    See all TNS4 primary antibodies
  • Description
    Mouse monoclonal to TNS4
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-481 of Human TNS4

Properties

Applications

Our Abpromise guarantee covers the use of ab57940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 20390342
Flow Cyt Use 0.01-0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMay be involved in cell migration, cartilage development and in linking signal transduction pathways to the cytoskeleton (By similarity). May promote apoptosis, via its cleavage by caspase-3.
  • Tissue specificityProstate and placenta. Down regulated in prostate cancer.
  • Sequence similaritiesContains 1 phosphatase tensin-type domain.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Proteolytically cleaved by caspase-3 during apoptosis.
  • Cellular localizationCell junction > focal adhesion. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • C terminal tensin like antibody
    • C terminal tensin like protein antibody
    • C-terminal tensin-like protein antibody
    • CTEN antibody
    • FLJ14950 antibody
    • PP14434 antibody
    • TENS4_HUMAN antibody
    • Tensin 4 antibody
    • Tensin-4 antibody
    • Tns4 antibody
    see all

Anti-TNS4 antibody images

  • Overlay histogram showing HeLa cells stained with ab57940 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57940, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Western blot against tagged recombinant protein immunogen using ab57940 TNS4 antibody at 1ug/ml. Predicted band size of immunogen is 79 kDa
  • ICC/IF image of ab57940 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57940, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-TNS4 antibody (ab57940)

This product has been referenced in:
  • Albasri A  et al. Nuclear expression of phosphorylated focal adhesion kinase is associated with poor prognosis in human colorectal cancer. Anticancer Res 34:3969-74 (2014). Read more (PubMed: 25075018) »
  • Albasri A  et al. CTEN (C-terminal tensin-like), a novel oncogene overexpressed in invasive breast carcinoma of poor prognosis. Breast Cancer Res Treat 126:47-54 (2011). IHC-P ; Human . Read more (PubMed: 20390342) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (mammary epithelial (MCF10A))
Loading amount 20 µg
Specification mammary epithelial (MCF10A)
Treatment 0-100nM EGF 24 hrs
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 20°C
Username

Dr. Jason Neil

Verified customer

Submitted Jan 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"