Overview

  • Product name
    Anti-TOB antibody
  • Description
    Mouse polyclonal to TOB
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human TOB (amino acids 1-345; NP_005740.1).

  • Positive control
    • HeLa cells; TOB transfected 293T cell lysate; Human endometrium tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab168947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Relevance
    The Tob/Btg family of proteins consists of a large number of members. These proteins have a common domain in their amino terminal end and may have anti-proliferative activity in various cell types. The Tob protein was identified in a search for molecules that interact with the receptor tyrosine kinase ErbB2. Active ErbB2 has a negative effect on the anti-proliferative activity of Tob. However, Tob is phosphorylated on serine and threonine residues but not on tyrosine, suggesting that active ErbB2 activates a Ser/Thr kinase that phosphorylates Tob. Unphosphorylated Tob suppresses expression of cyclin D1. It was shown that active p90Rsk1 kinase (known to be activated by protein-tyrosine kinase receptor) phosphorylates Tob on serine and threonine residues in vitro. In addition, Erk1/Erk2 MAP kinases phosphorylate Tob in vivo and in vitro, resulting in suppression of the anti-proliferative activity of Tob. Homozygous Tob knockout mice develop greater bone mass resulting from increased numbers of osteoblasts. Furthermore, it has been shown in osteoblasts, that upon BMP2 (bone morphogenetic protein) activation, Tob associates with receptor regulated Smads (Smad 1, 5, and 8). Thus, osteoblast proliferation and differentiation is negatively regulated by Tob protein through the Smad proteins.
  • Database links
  • Alternative names
    • APRO 6 antibody
    • APRO6 antibody
    • MGC104792 antibody
    • MGC34446 antibody
    • PIG 49 antibody
    • PIG49 antibody
    • Proliferation inducing gene 49 antibody
    • Protein Tob1 antibody
    • RP23 244C22.1 antibody
    • TOB 1 antibody
    • Tob 1 protein antibody
    • TOB antibody
    • TOB1 antibody
    • Tob1 protein antibody
    • Transducer of ERBB 2 1 antibody
    • Transducer of erbB 2 antibody
    • Transducer of ERBB2 1 antibody
    • Transducer of erbB2 antibody
    • Trob antibody
    • TROB 1 antibody
    • TROB1 antibody
    see all

Images

  • All lanes : Anti-TOB antibody (ab168947) at 1 µg/ml

    Lane 1 : TOB transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Developed using the ECL technique.

    Predicted band size: 38 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human endometrium tissue labeling TOB with ab168947 at 3 µg/ml.
  • Immunofluorescent analysis of HeLa cells labeling TOB with ab168947 at 10 µg/ml.

References

This product has been referenced in:
  • Guan R  et al. Decreased TOB1 expression and increased phosphorylation of nuclear TOB1 promotes gastric cancer. Oncotarget 8:75243-75253 (2017). Read more (PubMed: 29088861) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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