• Product name
    Anti-TOB (phospho S164) antibody
    See all TOB primary antibodies
  • Description
    Rabbit polyclonal to TOB (phospho S164)
  • Specificity
    ab78915 detects endogenous levels of TOB only when phosphorylated at serine 164.
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human TOB around the phosphorylation site of serine 164 (A-V-SP-P-T).

  • Positive control
    • Human brain tissue; HT-29 cell extracts, treated with serum (20%, 15mins).



Our Abpromise guarantee covers the use of ab78915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
IHC-P 1/50 - 1/100.


  • Relevance
    The Tob/Btg family of proteins consists of a large number of members. These proteins have a common domain in their amino terminal end and may have anti-proliferative activity in various cell types. The Tob protein was identified in a search for molecules that interact with the receptor tyrosine kinase ErbB2. Active ErbB2 has a negative effect on the anti-proliferative activity of Tob. However, Tob is phosphorylated on serine and threonine residues but not on tyrosine, suggesting that active ErbB2 activates a Ser/Thr kinase that phosphorylates Tob. Unphosphorylated Tob suppresses expression of cyclin D1. It was shown that active p90Rsk1 kinase (known to be activated by protein-tyrosine kinase receptor) phosphorylates Tob on serine and threonine residues in vitro. In addition, Erk1/Erk2 MAP kinases phosphorylate Tob in vivo and in vitro, resulting in suppression of the anti-proliferative activity of Tob. Homozygous Tob knockout mice develop greater bone mass resulting from increased numbers of osteoblasts. Furthermore, it has been shown in osteoblasts, that upon BMP2 (bone morphogenetic protein) activation, Tob associates with receptor regulated Smads (Smad 1, 5, and 8). Thus, osteoblast proliferation and differentiation is negatively regulated by Tob protein through the Smad proteins.
  • Database links
  • Alternative names
    • APRO 6 antibody
    • APRO6 antibody
    • MGC104792 antibody
    • MGC34446 antibody
    • PIG 49 antibody
    • PIG49 antibody
    • Proliferation inducing gene 49 antibody
    • Protein Tob1 antibody
    • RP23 244C22.1 antibody
    • TOB 1 antibody
    • Tob 1 protein antibody
    • TOB antibody
    • TOB1 antibody
    • Tob1 protein antibody
    • Transducer of ERBB 2 1 antibody
    • Transducer of erbB 2 antibody
    • Transducer of ERBB2 1 antibody
    • Transducer of erbB2 antibody
    • Trob antibody
    • TROB 1 antibody
    • TROB1 antibody
    see all

Anti-TOB (phospho S164) antibody images

  • ab78915 at 1/50 dilution staining TOB in human brain by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
  • All lanes : Anti-TOB (phospho S164) antibody (ab78915) at 1/500 dilution

    Lane 1 : HT-29 cell extracts, treated with serum (20%, 15mins)
    Lane 2 : HT-29 cell extracts, treated with serum (20%, 15mins) with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa

References for Anti-TOB (phospho S164) antibody (ab78915)

This product has been referenced in:
  • Zhang YW  et al. Acquisition of estrogen independence induces TOB1-related mechanisms supporting breast cancer cell proliferation. Oncogene 35:1643-56 (2016). Read more (PubMed: 26165839) »

See 1 Publication for this product

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