Overview

  • Product name
  • Description
    Rabbit polyclonal to TOE1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 249 and 445 of TOE1

  • Positive control
    • A549, HeLa, HepG2 and HCT116 whole cell lysate; HeLa cells; Colon cancer tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. Mediates the inhibitory growth effect of EGR1.
  • Sequence similarities
    Belongs to the CAF1 family.
    Contains 1 C3H1-type zinc finger.
  • Cellular localization
    Nucleus, nucleolus. Nucleus speckle. Localizes to nuclear speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • Target of EGR1 protein 1 antibody
    • target of EGR1, member 1 antibody
    • Toe1 antibody
    • TOE1_HUMAN antibody

Images

  • Anti-TOE1 antibody (ab151600) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size : 56 kDa
  • Immunofluorescence analysis using confocal microscopy of paraformaldehyde-fixed HeLa, labeling TOE1 using ab151600 at 1/500 dilution (Green). Alpha-tubulin filaments were labeled Red.
  • Immunohistochemical analysis of paraffin-embedded colon caner, labeling TOE1 using ab151600 at 1/250 dilution.

References

ab151600 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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