Overview

  • Product nameTolbutamide
  • Description
    K+ channel blocker
  • Biological descriptionBlocker of ATP-sensitive (KIR6.x) inward rectifier K+ channels.
  • Purity> 99%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120278 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Tolbutamide images

    • U-87 MG cells were incubated at 37°C for 30 minutes with vehicle control (0 µM) and 200 µM of tolbutamide (ab120278) in DMSO. Increased expression of hexokinase type II (ab70360) correlates with an increase in tolbutamide concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab70360 at 2 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

    References for Tolbutamide (ab120278)

    This product has been referenced in:
    • Guide to Receptors and Channels (GRAC), 4th Edition. Br J Pharmacol 158 Suppl 1:S1-254 (2009). Read more (PubMed: 19883461) »
    • Kubo Y  et al. International Union of Pharmacology. LIV. Nomenclature and molecular relationships of inwardly rectifying potassium channels. Pharmacol Rev 57:509-26 (2005). Read more (PubMed: 16382105) »

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