Overview

  • Product nameAnti-TOM1 antibody
    See all TOM1 primary antibodies
  • Description
    Mouse monoclonal to TOM1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Dog, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment: GLAGALDARQ QSTGAIPVTQ ACLMEDIEQW LSTDVGNDAE EPKGVTSEEF DKFLEERAKA ADRLPNLSSP SAEGPPGPPS GPAPRKKTQE KDDDMLFA, corresponding to amino acids 394-491 of Human TOM1 (NP_005479) with a proprietory tag.

  • Positive control
    • HeLa cell lysate. Recombinant tagged human TOM1 fragment.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab77649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged TOM1 is approximately 30ng/ml when used as a capture antibody.

Target

Anti-TOM1 antibody images

  • Anti-TOM1 antibody (ab77649) at 1 µg/ml + Recombinant tagged human TOM1 fragment at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 54 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Anti-TOM1 antibody (ab77649) at 1 µg/ml + HeLa cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 54 kDa
    Observed band size : 54 kDa

References for Anti-TOM1 antibody (ab77649)

ab77649 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"