Overview

  • Product name
  • Description
    Rabbit polyclonal to TOM1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 450-492 of Human TOM1 (NP_005479.1).

  • Positive control
    • HeLa and 293T whole cell lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab99356 was affinity purified using an epitope specific to TOM1 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Relevance
    Function: May be involved in intracellular trafficking. Probable association with membranes. Tissue specificity: Widely expressed. Highly expressed in skeletal muscle, heart, placenta and liver. Similarity: Belongs to the TOM1 family. Contains 1 GAT domain. Contains 1 VHS domain.
  • Cellular localization
    Cytoplasm. Membrane; Peripheral membrane protein
  • Database links
  • Alternative names
    • FLJ33404 antibody
    • OTTHUMP00000028777 antibody
    • Target of myb 1 antibody
    • Target of Myb protein 1 antibody
    • Target of myb1 antibody
    • TOM 1 antibody
    • TOM1 antibody
    • TOM1_HUMAN antibody
    see all

Images

  • All lanes : Anti-TOM1 antibody (ab99356) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 54 kDa


    Exposure time : 30 seconds
  • ab99356, at 1 µg/ml, detecting TOM1 in HeLa whole cell lysate by Western blot analysis following Immunoprecipitation (IP). Detection utilized ECL with a 3 second exposure.
    For immunoprecipitation, ab99356 was used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
    Lane 1; IP using ab99355 (a rabbit anti-TOM1 antibody which recognizes an upstream epitope).
    Lane 2; IP using ab99356.
    Lane 3; IP using control IgG.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab99356.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up