Overview

  • Product nameAnti-TOM1 antibody
    See all TOM1 primary antibodies
  • Description
    Rabbit polyclonal to TOM1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 450-492 of Human TOM1 (NP_005479.1).

  • Positive control
    • HeLa and 293T whole cell lysates

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab99356 was affinity purified using an epitope specific to TOM1 immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • RelevanceTOM1 may be involved in intracellular trafficking. In conjunction with Tollip it plays an important role in recruiting clathrin onto endosomes and may modulate endosomal functions.
  • Cellular localizationCytoplasm. Membrane; Peripheral membrane protein
  • Database links
  • Alternative names
    • FLJ33404 antibody
    • OTTHUMP00000028777 antibody
    • Target of myb 1 antibody
    • Target of Myb protein 1 antibody
    • Target of myb1 antibody
    • TOM 1 antibody
    see all

Anti-TOM1 antibody images

  • All lanes : Anti-TOM1 antibody (ab99356) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 54 kDa


    Exposure time : 30 seconds
  • ab99356, at 1 µg/ml, detecting TOM1 in HeLa whole cell lysate by Western blot analysis following Immunoprecipitation (IP). Detection utilized ECL with a 3 second exposure.
    For immunoprecipitation, ab99356 was used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
    Lane 1; IP using ab99355 (a rabbit anti-TOM1 antibody which recognizes an upstream epitope).
    Lane 2; IP using ab99356.
    Lane 3; IP using control IgG.

References for Anti-TOM1 antibody (ab99356)

ab99356 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"