Function: May be involved in intracellular trafficking. Probable association with membranes.
Tissue specificity: Widely expressed. Highly expressed in skeletal muscle, heart, placenta and liver.
Similarity: Belongs to the TOM1 family.
Contains 1 GAT domain.
Contains 1 VHS domain.
All lanes : Anti-TOM1 antibody (ab99356) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 54 kDa
Exposure time : 30 seconds
Immunoprecipitation - TOM1 antibody (ab99356)
ab99356, at 1 µg/ml, detecting TOM1 in HeLa whole cell lysate by Western blot analysis following Immunoprecipitation (IP). Detection utilized ECL with a 3 second exposure.
For immunoprecipitation, ab99356 was used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
Lane 1; IP using ab99355 (a rabbit anti-TOM1 antibody which recognizes an upstream epitope).
Lane 2; IP using ab99356.
Lane 3; IP using control IgG.