The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: antibody detection limit dilution 1/64000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 53 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionProbable adapter protein involved in signaling pathways. Interacts with the SH2 and SH3 domains of various signaling proteins when it is phosphorylated. May promotes FYN activation, possibly by disrupting intramolecular SH3-dependent interactions.
Sequence similaritiesBelongs to the TOM1 family. Contains 1 GAT domain. Contains 1 VHS domain.
Post-translational modificationsPhosphorylated on tyrosines by FYN (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.