The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: antibody detection limit dilution 1/64000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 53 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Probable adapter protein involved in signaling pathways. Interacts with the SH2 and SH3 domains of various signaling proteins when it is phosphorylated. May promotes FYN activation, possibly by disrupting intramolecular SH3-dependent interactions.
Belongs to the TOM1 family. Contains 1 GAT domain. Contains 1 VHS domain.
Phosphorylated on tyrosines by FYN (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.