Overview

  • Product name
  • Description
    Rabbit polyclonal to TOM1L2
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen sequence, corresponding to amino acids 158-242 (ALSPIHTPQR SVPEVDPAAT MPRSQSQQRT SAGSYSSPPP APYSAPQAPA LSVTGPITAN SEQIARLRSE LDVVRGNTKV MSEML) of Human TOM1L2.

  • Positive control
    • Human heart muscle tissue; RT4, U251 MG, Human plasma, liver and tonsil lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nuclei but not nucleoli. Recommended concentration of ab121716 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • ab121716 at 1/250 dilution staining TOM1L2 in Paraffin-embedded Human heart muscle tissue by Immunohistochemistry.
  • All lanes : Anti-TOM1L2 antibody (ab121716) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U251 MG cell lysate
    Lane 3 : Human Plasma
    Lane 4 : Human Liver tissue lysate
    Lane 5 : Human Tonsil tissue lysate

    Developed using the ECL technique
  • Immunohistochemical staining of Human heart muscle shows moderate cytoplasmic positivity in myocytes using ab121716

References

ab121716 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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