Overview

  • Product name
  • Description
    Goat polyclonal to TOM1L2
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Dog
  • Immunogen

    Synthetic peptide:

    (C)RKKPERSEDALFAL

    , corresponding to C terminal amino acids 462-475 of Human TOM1L2.

  • Positive control
    • Human Fibroblast A431 lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 60 kDa.

Target

  • Function
    Probable role in protein transport. May regulate growth factor-induced mitogenic signaling.
  • Tissue specificity
    Ubiquitously expressed with higher expression in heart and skeletal muscle.
  • Sequence similarities
    Belongs to the TOM1 family.
    Contains 1 GAT domain.
    Contains 1 VHS domain.
  • Domain
    The GAT domain mediates interaction with TOLLIP.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ32746 antibody
    • Srebf1 antibody
    • Target of Myb-like protein 2 antibody
    • Target of myb1 like 2 antibody
    • Target of myb1-like 2 (chicken) antibody
    • TM1L2_HUMAN antibody
    • TOM1 antibody
    • TOM1-like 2 antibody
    • TOM1-like protein 2 antibody
    • TOM1L 2 antibody
    • Tom1l2 antibody
    see all

Images

  • ab24369 (5µg/ml) staining of TOM1L2 if paraffin embedded Human Skeletal Muscle with steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows variable staining from fibre to fibre.

  • Anti-TOM1L2 antibody (ab24369) at 0.03 µg/ml + A431 lysate (35µg protein in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References

ab24369 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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