Recombinant Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15581-39] to TOMM20 - Mitochondrial Marker
- Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker
See all TOMM20 primary antibodies -
Description
Rabbit monoclonal [EPR15581-39] to TOMM20 - Mitochondrial Marker -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2 and HeLa whole cell lysate (ab150035). IHC-P: Human endometrial adenocarcinoma, human cervical cancer, mouse cardiac muscle tissues, rat testis, mouse colon. ICC/IF: HeLa cells. Flow Cyt (intra): NCI-H2228 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15581-39 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205486)
- Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205487)
- HRP Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab205796)
- Alexa Fluor® 555 Anti-TOMM20 antibody [EPR15581-39] (ab214409)
- Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (ab220822)
- Anti-TOMM20 antibody [EPR15581-39] - Mouse IgG1 (Chimeric) (ab283317)
- Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (ab289670)
- Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-39] - Mouse IgG1 (ab309166)
- Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (ab309167)
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/40.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P | (1) |
1/50 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/250 - 1/500.
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WB | (5) |
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
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Notes |
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Flow Cyt (Intra)
1/40. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/50 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
WB
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa). |
Target
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Function
Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. -
Sequence similarities
Belongs to the Tom20 family. -
Cellular localization
Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 9804 Human
- Entrez Gene: 67952 Mouse
- Entrez Gene: 266601 Rat
- Omim: 601848 Human
- SwissProt: Q15388 Human
- SwissProt: Q9DCC8 Mouse
- SwissProt: Q62760 Rat
- Unigene: 533192 Human
see all -
Alternative names
- KIAA0016 antibody
- MAS20 antibody
- MGC117367 antibody
see all
Images
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Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734) at 1/5000 dilution + HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa -
Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cells) labeling TOMM20 with ab186734 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/100 was used as counterstain antibody.
Confocal image showing cytoplasmic staining in HeLa cells.
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Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue, labeling TOMM20 with ab186734 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).
Granular cytoplasmic staining on human cervical cancer.
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Intracellular Flow Cytometry analysis of TOMM20 expression in NCI-H2228 (Human lung cancer cell line) cells using ab186734 at 1/40 dilution (pink) and a rabbit IgG as negative control (green).
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Immunohistochemical analysis of paraffin-embedded mouse colon tissue, labeling TOMM20 with ab186734 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).
Granular cytoplasmic staining on mouse colon.
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AltMiD515’ expression induces mitochondrial fission.
Confocal microscopy of mock-transfected cells, cells transfected with altMiD51WT, altMiD51LYR→AAA or Drp1K38A immunostained with anti-TOM20 (red channel) and anti-Flag (green channel) monoclonal antibodies. In each image, boxed areas are shown at higher magnification in the bottom right corner.
TOMM20 is detected using ab186734 at 1/500 dilution.
From Figure 12c of Samandi et al.
Samandi et al eLife. 2017; 6: e27860. Published online 2017 Oct 30. doi: 10.7554/eLife.27860
Reproduced underthe Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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Immunofluorescence analysis of paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, labeling TOMM20 (green) with ab186734 at 1/250 dilution.
Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded rat testis tissue, labeling TOMM20 with ab186734 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).
Granular cytoplasmic staining on rat testis.
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Immunohistochemical analysis of paraffin-embedded human endometrial adenocarcinoma tissue, labeling TOMM20 with ab186734 at 1/100 dilution.
Detected using HRP polymer for Rabbit IgG and counterstained using hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling TOMM20 with ab186734 at 1/100 dilution.
Detected using HRP polymer for Rabbit IgG and counterstained using hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734) at 1/20000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (79)
ab186734 has been referenced in 79 publications.
- van Beek SMM et al. Effect of β2-agonist treatment on insulin-stimulated peripheral glucose disposal in healthy men in a randomised placebo-controlled trial. Nat Commun 14:173 (2023). PubMed: 36635304
- Laporte MH et al. Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM). Nat Methods 19:216-222 (2022). PubMed: 35027766
- Yang J et al. Pitavastatin activates mitophagy to protect EPC proliferation through a calcium-dependent CAMK1-PINK1 pathway in atherosclerotic mice. Commun Biol 5:124 (2022). PubMed: 35145192
- Kumar R et al. A cell-based GEF assay reveals new substrates for DENN domains and a role for DENND2B in primary ciliogenesis. Sci Adv 8:eabk3088 (2022). PubMed: 35196081
- Ball JM et al. Mitochondria in cone photoreceptors act as microlenses to enhance photon delivery and confer directional sensitivity to light. Sci Adv 8:eabn2070 (2022). PubMed: 35235359