Overview

  • Product nameAnti-TOMM22 antibody [1C9-2]
    See all TOMM22 primary antibodies
  • Description
    Mouse monoclonal [1C9-2] to TOMM22
  • Tested applicationsSuitable for: ICC, WBmore details
  • Species reactivity
    Reacts with: Human, Monkey
  • Immunogen

    Membrane fraction from Vero (monkey kidney-derived) cells.

  • Positive control
    • Raji whole cell lysate.
  • General notes


    Tom22 has a negatively charged N-terminal region exposed to the cytosol, a putative transmembrane region, and a C-terminal intermembrane space region with little negative charge. The following functions can be assigned to the three domains of Tom22: the cytosolic N-terminal domain plays a dual role, specifically important for presequence binding; the intermembrane space domain provides a trans binding site for presequences, and the single membrane anchor of Tom22 is crucial for the integrity of the GIP (general import gene) complex. In the absence of this membrane anchor, the GIP complex dissociates into small core complexes containing a dimer of Tom40 and the three small Toms. A preprotein is stably arrested and accumulated in the GIP complex by Tom40 and Tom22.4 Such a 100 kDa core complex probably contains a single channel that retains the basic channel properties but is already open in the absence of preproteins. In contrast, in the presence of Tom22, the wild-type GIP complex contains tightly regulated channels (probably three channels). Tom22 apparently represents a component of the machinery that controls the gate. The cytosolic domains of Tom22 and Tom20 are believed to form the major part of a cis site, which mediates the import of all preproteins known to use the general import machinery of mitochondria. The preprotein is then routed through the Tom complex translocation channel and transferred to a trans site on the intermembrane space (IMS) side of the outer membrane. The inter-membrane space exposed segment of Tom40 and the C-terminal tail of Tom22 may contribute to the trans-site. Matrix-targeted proteins are further transferred to the matrix through Product Information import machinery in the inner membrane. The TOM complex of mammalian mitochondria resembles the fungal Tom complex, but is distinct from the plant TOM system. Thus, while unique components of the mammalian mitochondrial import system have been identified (e.g. TOM34 and metaxin), Tom22, and Tom37 have not been identified in plant mitochondria.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 1% BSA, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesTom22 has a negatively charged N-terminal region exposed to the cytosol, a putative transmembrane region, and a C-terminal intermembrane space region with little negative charge. The following functions can be assigned to the three domains of Tom22: the cytosolic N-terminal domain plays a dual role, specifically important for presequence binding; the intermembrane space domain provides a trans binding site for presequences, and the single membrane anchor of Tom22 is crucial for the integrity of the GIP (general import gene) complex. In the absence of this membrane anchor, the GIP complex dissociates into small core complexes containing a dimer of Tom40 and the three small Toms. A preprotein is stably arrested and accumulated in the GIP complex by Tom40 and Tom22.4 Such a 100 kDa core complex probably contains a single channel that retains the basic channel properties but is already open in the absence of preproteins. In contrast, in the presence of Tom22, the wild-type GIP complex contains tightly regulated channels (probably three channels). Tom22 apparently represents a component of the machinery that controls the gate. The cytosolic domains of Tom22 and Tom20 are believed to form the major part of a cis site, which mediates the import of all preproteins known to use the general import machinery of mitochondria. The preprotein is then routed through the Tom complex translocation channel and transferred to a trans site on the intermembrane space (IMS) side of the outer membrane. The inter-membrane space exposed segment of Tom40 and the C-terminal tail of Tom22 may contribute to the trans-site. Matrix-targeted proteins are further transferred to the matrix through Product Information import machinery in the inner membrane. The TOM complex of mammalian mitochondria resembles the fungal Tom complex, but is distinct from the plant TOM system. Thus, while unique components of the mammalian mitochondrial import system have been identified (e.g. TOM34 and metaxin), Tom22, and Tom37 have not been identified in plant mitochondria.
  • ClonalityMonoclonal
  • Clone number1C9-2
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 22 kDa.

Target

  • Cellular localizationMitochondrion outer membrane; Single-pass membrane protein
  • Database links
  • Alternative names
    • 1C9 2 antibody
    • hTom 22 antibody
    • hTom22 antibody
    • Mitochondrial import receptor subunit TOM 22 homolog antibody
    • Mitochondrial import receptor subunit TOM22 homolog antibody
    • Mitochondrial import receptor Tom 22 antibody
    • Mitochondrial import receptor Tom22 antibody
    • MST 065 antibody
    • MST065 antibody
    • MSTP 065 antibody
    • MSTP065 antibody
    • TOM 22 antibody
    • TOM22 antibody
    • TOMM 22 antibody
    • Translocase of outer membrane 22 kDa subunit homolog antibody
    • Translocase of outer mitochondrial membrane 22 homolog antibody
    see all

References for Anti-TOMM22 antibody [1C9-2] (ab10436)

This product has been referenced in:
  • Gammage PA  et al. Near-complete elimination of mutant mtDNA by iterative or dynamic dose-controlled treatment with mtZFNs. Nucleic Acids Res 44:7804-16 (2016). WB, ICC/IF ; Human . Read more (PubMed: 27466392) »
  • Gammage PA  et al. Mitochondrially targeted ZFNs for selective degradation of pathogenic mitochondrial genomes bearing large-scale deletions or point mutations. EMBO Mol Med 6:458-66 (2014). WB . Read more (PubMed: 24567072) »

See all 5 Publications for this product

Product Wall

Thank you for the information. I will forward this along to Bill, as he is out of the office this week. If you have a few moments, would you be interested in writing an Abreview for this product? In return for writing the review and letting future...

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Thank you for your enquiry and for sending us your detailed protocol. We are very sorry to hear that you are not satisfied with this antibody. We would like to make some comments and/or suggestions: 1. This antibody has been characterised on hum...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"